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Alberta NINT/29 May 2008
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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| + | [[Alberta_NINT/28_May_2008 | < Previous entry]] | [[Alberta_NINT/30_May_2008 | Next entry >]] | ||
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lab work (SD): | lab work (SD): | ||
| - | Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). | + | Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) |
| + | (plates from 28/05/09). | ||
DNA was run on 2% agarose gel. All 9 samples were identical on the gel. | DNA was run on 2% agarose gel. All 9 samples were identical on the gel. | ||
| - | K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random | + | K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were |
| - | + | selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C. | |
| + | [[Image:NINT_SD_p19.jpg|500px]] | ||
lab work (JD): | lab work (JD): | ||
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Transformed XL1-B cells with K102001. | Transformed XL1-B cells with K102001. | ||
Prepared R0011 for sequencing and dropped it off. | Prepared R0011 for sequencing and dropped it off. | ||
| + | [[Image:slide1.jpg|750px]] | ||
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| + | lab work (WM): | ||
| + | # colonies from K102010 ligation: ~100 negative ctl: 0 | ||
| + | setup 8 O/Ns | ||
Latest revision as of 22:05, 11 October 2008
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lab work (SD):
Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B)
(plates from 28/05/09).
DNA was run on 2% agarose gel. All 9 samples were identical on the gel.
K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were
selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
lab work (JD):
Gel purified R0011 band from gel electrophoresis.
Carried out quantitative analysis
Carried out ligation to create K102001 (R0011+T/A1)
Transformed XL1-B cells with K102001.
Prepared R0011 for sequencing and dropped it off.
lab work (WM):
# colonies from K102010 ligation: ~100 negative ctl: 0
setup 8 O/Ns
