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Team:Newcastle University/Protocols
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{{:Team:Newcastle University/Template:UnderTheWetLab|page-title=[[Team:Newcastle University/Protocols|Protocols]]}} | {{:Team:Newcastle University/Template:UnderTheWetLab|page-title=[[Team:Newcastle University/Protocols|Protocols]]}} | ||
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| - | + | ==Protocols== | |
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[[Agarose Gel Electrophoresis]] | [[Agarose Gel Electrophoresis]] | ||
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[[Restricting Plasmids (Double Restriction)]] | [[Restricting Plasmids (Double Restriction)]] | ||
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| + | [[Purifying DNA from an enzymatic reaction]] | ||
[[Cutting a Specific Band from Agarose Gel]] | [[Cutting a Specific Band from Agarose Gel]] | ||
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[[Ligating DNA]] | [[Ligating DNA]] | ||
| - | [[Transforming into DH5α E. coli]] | + | [[Transforming into DH5α or TOP10 E. coli]] |
[[Making Frozen Glycerol Cell Stocks From Overnight Cultures]] | [[Making Frozen Glycerol Cell Stocks From Overnight Cultures]] | ||
[[Making Overnight Cultures from Frozen Glycerol Cell Stocks]] | [[Making Overnight Cultures from Frozen Glycerol Cell Stocks]] | ||
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| + | [[Making Overnight Cultures from Agar Colonies]] | ||
[[Transforming into Bacillus subtillis]] | [[Transforming into Bacillus subtillis]] | ||
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| + | [[Inducing Bacillus subtilis with Subtilin]] | ||
[[Preparing Bacillus subtilis for Microscopy]] | [[Preparing Bacillus subtilis for Microscopy]] | ||
| + | </div> | ||
Latest revision as of 23:03, 30 September 2008
Newcastle University
GOLD MEDAL WINNER 2008
| Home | Team | Original Aims | Software | Modelling | Proof of Concept Brick | Wet Lab | Conclusions |
|---|
Protocols
Isolating Plasmid from Cells (Miniprep)
Restricting Plasmids (Double Restriction)
Purifying DNA from an enzymatic reaction
Cutting a Specific Band from Agarose Gel
Transforming into DH5α or TOP10 E. coli
Making Frozen Glycerol Cell Stocks From Overnight Cultures
Making Overnight Cultures from Frozen Glycerol Cell Stocks
Making Overnight Cultures from Agar Colonies
Transforming into Bacillus subtillis
