"
Making Overnight Cultures from Agar Colonies
From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
| Home | Team | Original Aims | Software | Modelling | Proof of Concept Brick | Wet Lab | Conclusions |
|---|
Home >> Wet Lab >> Protocols >> Making Overnight Cultures from Agar Colonies
- Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
- Using an autoclaved toothpick, gently touch the point to a single colony.
- Drop the toothpick point down into the LB.
- Incubate overnight at 37°C whilst shaking.
