Team:Hawaii/Notebook/2008-09-19

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BB-pRL1383a=== :<strong> Grace</strong> ===Construction of secretion device=== :<strong>Grace</strong> ...)
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:::* pilA
:::* pilA
:::* slr2
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::* EcoRI/PstI
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:::* pSB1A3
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== Drylab Work ==
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:<strong> Krystle </strong>
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===Name of Task===
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:*Transformation of overnight ligations
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:<strong> name of person/people who performed the task</strong>
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===Gel to confirm transformants===
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<strong>Margaret</strong>
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:*
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
 
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:* e.g. read through papers, worked on proposal, etc.
 

Latest revision as of 01:37, 21 September 2008

Projects Events Resources
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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep of BB-pRL1383a

Grace

Construction of secretion device

Grace
  • PCR of nir+rbs, rbs+GFP, slr1+GFPf, pilA, slr2
  • Overnight RE digest:
  • EcoRI/SpeI
  • nir+rbs
  • rbs+GFP
  • slr1+GFPf
  • nir (PCR product from before)
  • XbaI/PstI
  • rbs+GFP
  • slr1+GFPf
  • pilA
  • slr2
  • EcoRI/PstI
  • pSB1A3
Krystle
  • Transformation of overnight ligations

Gel to confirm transformants

Margaret


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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