Latest revision as of 01:06, 23 September 2008

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Things we did today

Margaret

Included in my last transformation were pSB1A3, de-phosphorylated pSB1A3, and restriction digested (E,P) pSB1A3. All had colonies on DH5 alpha, and I want to see what they are.
Colony PCR, 62°C annealing, 1 minute extension.
Run 2%gel to compare them to colonies of curious size. I will also run the 8 rep colonies from this transformation that I ran a PCR on yesterday.
PCR order: 2 pSB1A3, restriction digested (E,P) pSB1A3, & 2 de-phosphorylated pSB1A3, (+) pSB1A3 from a plasmid prep, and (-) water. All using VF2&VR primers.

Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 9: / Line 9: @@
 :*Colony PCR, 62&deg;C annealing, 1 minute extension.
 :*Run 2%gel to compare them to colonies of curious size. I will also run the 8 rep colonies from this transformation that I ran a PCR on yesterday.
+:*PCR order: 2 pSB1A3, restriction digested (E,P) pSB1A3, & 2 de-phosphorylated pSB1A3, (+) pSB1A3 from a plasmid prep, and (-) water. All using VF2&VR primers.
-== Drylab Work ==
-===Name of Task===
+===Transformation of ligations into DH5&alpha;===
-:<strong> name of person/people who performed the task</strong>
+:<strong> Grace</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. read through papers, worked on proposal, etc.
+:* Used 5&mu;l of [[Team:Hawaii/Notebook/2008-09-20|ligation reaction]] from yesterday
 = Discussion =