Team:NTU-Singapore/Notebook/2 June 2008

From 2008.igem.org

(Difference between revisions)
m (New page: =Monday 2nd June= ==Hung(~~~), Zhen Fu== *)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
 +
<html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html>
 +
 +
<div id="header">{{User:Greenbear/sandbox/header}}</div>
 +
 +
<div id="maincontent" style="margin-top:130px;">
 +
<html>
 +
<div id="arrow">
 +
<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
 +
"
 +
    alt="Back to Notebook"
 +
    title="Back to Notebook">
 +
</a>
 +
</div>
 +
</html>
 +
=Monday 2nd June=
=Monday 2nd June=
-
==Hung([[User:Greenbear|Greenbear]]), Zhen Fu==
+
<div class="quote" style="margin:20px;">
-
*
+
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
 +
|
 +
===Personnel===
 +
Hung([[User:Greenbear|Greenbear]]), Zhen Fu
 +
===Experiment===
 +
**Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
 +
**#1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
 +
**#1 LB plate, half for BW, half for W3110
 +
**Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
 +
**Overnight incubation at 37 degrees C of these 3 agar plates:
 +
**#LB AmpR spread with 250ul cells with LacI-GFP plasmid.
 +
**#LB AmpR spread with 125ul cells with Terminator plasmid.
 +
**#LB KanR spread with 125ul cells with Terminator plasmid.
 +
**Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
 +
**#2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
 +
**#2 tubes each containing 5ml LB broth without Ampicilin.
 +
</div>

Latest revision as of 00:27, 28 October 2008

Monday 2nd June

Personnel

Hung(Greenbear), Zhen Fu

Experiment

    • Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
      1. 1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
      2. 1 LB plate, half for BW, half for W3110
    • Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
    • Overnight incubation at 37 degrees C of these 3 agar plates:
      1. LB AmpR spread with 250ul cells with LacI-GFP plasmid.
      2. LB AmpR spread with 125ul cells with Terminator plasmid.
      3. LB KanR spread with 125ul cells with Terminator plasmid.
    • Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
      1. 2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
      2. 2 tubes each containing 5ml LB broth without Ampicilin.
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/2_June_2008"