Alberta NINT/29 May 2008

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(Difference between revisions)

Latest revision as of 22:05, 11 October 2008

lab work (SD):

          Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B)
          (plates from 28/05/09).  
          DNA was run on 2% agarose gel.  All 9 samples were identical on the gel. 
          K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were      
          selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.

lab work (JD):

         Gel purified R0011 band from gel electrophoresis. 
         Carried out quantitative analysis  
         Carried out ligation to create K102001 (R0011+T/A1)
         Transformed XL1-B cells with K102001. 
         Prepared R0011 for sequencing and dropped it off.

lab work (WM):

         # colonies from K102010 ligation: ~100        negative ctl: 0
         setup 8 O/Ns

@@ Line 1: / Line 1: @@
+[[Team:Alberta_NINT/Notebook | < Back to notebook]]
+[[Alberta_NINT/28_May_2008 | < Previous entry]] | [[Alberta_NINT/30_May_2008 | Next entry >]]
 lab work (SD):
@@ Line 6: / Line 11: @@
             K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were
             selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
+          [[Image:NINT_SD_p19.jpg|500px]]
 lab work (JD):
@@ Line 14: / Line 20: @@
            Transformed XL1-B cells with K102001.
            Prepared R0011 for sequencing and dropped it off.
+          [[Image:slide1.jpg|750px]]
+lab work (WM):
+          # colonies from K102010 ligation: ~100        negative ctl: 0
+          setup 8 O/Ns