Team:Warsaw/Calendar-Main/16 July 2008

From 2008.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3>
 +
<h4>Michał L., Ewa, Marcin</h4>
 +
<p>We have massive outbreak of some virulent bacteriophage strain in our lab. All our current E. coli cultures are gone :-(. Until we get rid of the phage there will be no microbiological work in our lab. Sorry.<p/>
-
 
+
<h3>Cloning of protein Z DNA to OmpA constructs</h3><h4>Michał K.</h4>
-
<h3>Cloning of protein Z DNA to OmpA constructs</h3>
+
<p><ol>
<p><ol>
-
<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li>
-
<li> Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p>
+
<li> Control  
-
 
+
<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - both checked colones are good (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008#fig1">Fig. 1.</a>).</li>
-
 
+
</ol></p>
-
 
+
 +
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/a/ac/Dwa.jpg" width=300/></a> <var><b>Fig. 1. </b>Control SacI/BamHI digests of isolated plasmids<br>
 +
1. Marker<br>
 +
2-3. digested plasmids pACYC177+OmpA_Z_omega <br></var>
 +
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 17:55, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

We have massive outbreak of some virulent bacteriophage strain in our lab. All our current E. coli cultures are gone :-(. Until we get rid of the phage there will be no microbiological work in our lab. Sorry.

Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_omega).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
  3. Gel electrophoresis - both checked colones are good (Fig. 1.).

Fig. 1. Control SacI/BamHI digests of isolated plasmids
1. Marker
2-3. digested plasmids pACYC177+OmpA_Z_omega