Latest revision as of 04:33, 3 October 2008

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Grace

File:100208colonyPCR.png

EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.

Most lanes blank; lanes with bands are mostly faint. Didn't load enough PCR product? Will reload gel tomorrow with remainder of PCR product.

Margaret

Margaret

Margaret

ap1, and ap2. Because I need to digest them with E,S so that I can use as a front insert

Margaret

Margaret

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 46: / Line 46: @@
 |align=center|11
 |}
-:* Colony PCR of transformants
+:* Colony PCR of transformants\
+::* Most lanes blank; lanes with bands are mostly faint. Didn't load enough PCR product? Will reload gel tomorrow with remainder of PCR product.
 :* Restreaked:
+::* rbs+GFP+tt #9, 10, 16, 17
+::* slr1+GFPf+tt #13
+::* nir+rbs+slr1+GFPf #1, 2, 15, 17
+===Plasmid Prep===
+:<strong> Margaret</strong>
+:*oriV 3, 4, & 6
+===Restriction Digest===
+:<strong> Margaret </strong>
+:*oriV3 E,S
+:*oriV4 E,S
+:*oriV6 E,S
+:*oriV11 E,S
+:*plac/rbs/rep 8 X,P
+:*pSB1A3 E,P
+:*oriT E,S, and another sample of oriT with X,P
+===Culture===
+:<strong> Margaret </strong>
+:*ap1, and ap2. Because I need to digest them with E,S so that I can use as a front insert
+===PCR Verification===
+:<strong> Margaret </strong>
+:*10 more colonies of plac/rbs/rep
+:*check if SAP and SAP buffer is contaminated
+===Sequencing===
+:<strong> Margaret </strong>
+:*oriV 3, 4, 6
+:*plac/rbs/rep 8 with 11 primers to cover whole sequence