Team:Warsaw/Calendar-Main/9 May 2008

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Latest revision as of 16:32, 28 October 2008


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Preparation of pMPMT5+AID construct

Michał K.

Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). Fig. 1.
Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). Fig. 2.
Electrophoresis to estimate the concentration of isolated DNA.
Ligation of isolated DNA fragments.

Fig. 1. 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII

Fig. 2. 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII

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-<html><h3>Preparation of pMPMT5+AID construct<br>
+<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3>
-Michał K.</h3>
+<h4>Michał K.</h4>
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 <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</li>
-<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</li>
+<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008#fig1">Fig. 1</a>.</li>
-<img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=300/>
-<em>1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII</em>
 <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li>
-<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</li>
+<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008#fig2">Fig. 2</a>.</li>
-<img src="https://static.igem.org/mediawiki/2008/0/0f/Gel_out_pmpm_WAW.jpg" width=300/>
-<em>1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII</em>
 <li>Electrophoresis to estimate the concentration of isolated DNA.</li>
 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li>
 </ol></p>
-<div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div>
+<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=170/></a>
+<var><b>Fig. 1.</b> 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII</var>
+<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/0/0f/Gel_out_pmpm_WAW.jpg" width=200/></a>
+<var><b>Fig. 2.</b> 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII</var>
 </html>