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Team:Hawaii/Notebook/2008-06-10
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Name of Task=== :<strong> Grace </strong> :* Made LB+Kan50 plates :* Streaked out DH5α transformed with pRL1383a...) |
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= Things we did today = | = Things we did today = | ||
== Wetlab work == | == Wetlab work == | ||
| - | === | + | ===Made LB+Kan50 plates=== |
:<strong> Grace </strong> | :<strong> Grace </strong> | ||
:* Made LB+Kan50 plates | :* Made LB+Kan50 plates | ||
| + | |||
| + | ===Preparation for tri-parental conjugation=== | ||
| + | :<strong> Grace </strong> | ||
:* Streaked out DH5α transformed with pRL1383a on LB+sp for conjugation tomorrow | :* Streaked out DH5α transformed with pRL1383a on LB+sp for conjugation tomorrow | ||
:* Streaked out ''E. coli'' transformed with RP1 on LB+kan for conjugation tomorrow | :* Streaked out ''E. coli'' transformed with RP1 on LB+kan for conjugation tomorrow | ||
| - | :* Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. [Team:Hawaii/ | + | ===Biobrick extraction/transformation=== |
| - | + | :<strong> Grace </strong> | |
| + | :* Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. [[Team:Hawaii/Biobricks_extraction_%26_transformation_competency_test|(see experiment)]] | ||
:<strong> Norman </strong> | :<strong> Norman </strong> | ||
| Line 18: | Line 22: | ||
:* Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO<sub>2</sub> incubation | :* Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO<sub>2</sub> incubation | ||
:* Got ''Anabena'' triparental conjugation protocol from SC | :* Got ''Anabena'' triparental conjugation protocol from SC | ||
| + | :* Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/gene location. | ||
== Discussion == | == Discussion == | ||
| + | * Norman will figure out concentration of DNA on Biobricks filter paper | ||
| + | * Norman will make LB+amp100 plates tonight | ||
| + | * Need to check incubated plates tomorrow (Krystle?) | ||
| + | * Need to create growth curve (OD750 vs. cell population) | ||
| + | * Need to practice lifting agar for antibiotic insertion (Thursday?) | ||
== Quote of the Day == | == Quote of the Day == | ||
| - | + | <blockquote> ''As long as you don’t know what it is, you don’t need to fill out forms.''</blockquote> | |
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Latest revision as of 01:06, 12 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Made LB+Kan50 plates
- Grace
- Made LB+Kan50 plates
Preparation for tri-parental conjugation
- Grace
- Streaked out DH5α transformed with pRL1383a on LB+sp for conjugation tomorrow
- Streaked out E. coli transformed with RP1 on LB+kan for conjugation tomorrow
Biobrick extraction/transformation
- Grace
- Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. (see experiment)
- Norman
- Streaked out transformants from yesterday's BioBricks transformation/extraction
Drylab Work
- Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO2 incubation
- Got Anabena triparental conjugation protocol from SC
- Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/gene location.
Discussion
- Norman will figure out concentration of DNA on Biobricks filter paper
- Norman will make LB+amp100 plates tonight
- Need to check incubated plates tomorrow (Krystle?)
- Need to create growth curve (OD750 vs. cell population)
- Need to practice lifting agar for antibiotic insertion (Thursday?)
Quote of the Day
As long as you don’t know what it is, you don’t need to fill out forms.
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
