"
Team:Hawaii/Notebook/2008-06-10
From 2008.igem.org
(Difference between revisions)
(→Drylab Work) |
(→Quote of the Day) |
||
| (3 intermediate revisions not shown) | |||
| Line 14: | Line 14: | ||
===Biobrick extraction/transformation=== | ===Biobrick extraction/transformation=== | ||
:<strong> Grace </strong> | :<strong> Grace </strong> | ||
| - | :* Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. [[Team:Hawaii/ | + | :* Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. [[Team:Hawaii/Biobricks_extraction_%26_transformation_competency_test|(see experiment)]] |
:<strong> Norman </strong> | :<strong> Norman </strong> | ||
| Line 22: | Line 22: | ||
:* Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO<sub>2</sub> incubation | :* Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO<sub>2</sub> incubation | ||
:* Got ''Anabena'' triparental conjugation protocol from SC | :* Got ''Anabena'' triparental conjugation protocol from SC | ||
| - | :* Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/ | + | :* Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/gene location. |
== Discussion == | == Discussion == | ||
| + | * Norman will figure out concentration of DNA on Biobricks filter paper | ||
| + | * Norman will make LB+amp100 plates tonight | ||
| + | * Need to check incubated plates tomorrow (Krystle?) | ||
| + | * Need to create growth curve (OD750 vs. cell population) | ||
| + | * Need to practice lifting agar for antibiotic insertion (Thursday?) | ||
== Quote of the Day == | == Quote of the Day == | ||
| - | + | <blockquote> ''As long as you don’t know what it is, you don’t need to fill out forms.''</blockquote> | |
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Latest revision as of 01:06, 12 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Made LB+Kan50 plates
- Grace
- Made LB+Kan50 plates
Preparation for tri-parental conjugation
- Grace
- Streaked out DH5α transformed with pRL1383a on LB+sp for conjugation tomorrow
- Streaked out E. coli transformed with RP1 on LB+kan for conjugation tomorrow
Biobrick extraction/transformation
- Grace
- Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. (see experiment)
- Norman
- Streaked out transformants from yesterday's BioBricks transformation/extraction
Drylab Work
- Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO2 incubation
- Got Anabena triparental conjugation protocol from SC
- Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/gene location.
Discussion
- Norman will figure out concentration of DNA on Biobricks filter paper
- Norman will make LB+amp100 plates tonight
- Need to check incubated plates tomorrow (Krystle?)
- Need to create growth curve (OD750 vs. cell population)
- Need to practice lifting agar for antibiotic insertion (Thursday?)
Quote of the Day
As long as you don’t know what it is, you don’t need to fill out forms.
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
