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Team:Michigan/9 October 2008
From 2008.igem.org
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==<B> Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them </B>== | ==<B> Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them </B>== | ||
| - | *PCR clean-up (quenches digests) on our digests from yesterday | + | *[https://2008.igem.org/Team:Michigan/Notebook/PCRProtocol <u>PCR clean-up</u>] (quenches digests) on our digests from yesterday |
| - | *Attempted two ligations: pCLOCK1,nifA (NdeI/SpeI) and BBa R0010 (lacI inducible lac promoter), nifA (R0010 with SpeI, nifA with XbaI/SpeI); transformed onto Ampicillin plates | + | *Attempted two [https://2008.igem.org/Team:Michigan/Notebook/LigationProtocol <u>ligations</u>]: pCLOCK1,nifA (NdeI/SpeI) and BBa R0010 (lacI inducible lac promoter), nifA (R0010 with SpeI, nifA with XbaI/SpeI); [https://2008.igem.org/Team:Michigan/Notebook/TransformationProtocol <u>transformed</u>] onto Ampicillin plates |
| - | *Ran analytical gels on the digests too - all lengths matched up, except for our midi-prep of pCLOCK2; something messed up there, as the ladder in the lane next door didn't work either; need to re-check pCLOCK2 | + | *Ran [https://2008.igem.org/Team:Michigan/Notebook/GelProtocol <u>analytical gels</u>] on the digests too - all lengths matched up, except for our midi-prep of pCLOCK2; something messed up there, as the ladder in the lane next door didn't work either; need to re-check pCLOCK2 |
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| + | |} | ||
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| + | {|class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 1px; background-color:dodgerblue; border: 1px solid mediumblue; text-align:center" | ||
| + | !width="10%" align="left" valign="top" style="background:gold; color:black"| | ||
| + | <font color=navy> | ||
| + | |||
| + | == <font color=dodgerblue size=5> Calendar </font> == | ||
<table> | <table> | ||
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table.month .heading td { padding:1px; background-color: dodgerblue; color: navy; text-align:center; font-size:140%; font-weight:bold; } | table.month .heading td { padding:1px; background-color: dodgerblue; color: navy; text-align:center; font-size:140%; font-weight:bold; } | ||
table.month .dow td { color:navy; text-align:center; font-size:100%; } | table.month .dow td { color:navy; text-align:center; font-size:100%; } | ||
| - | table.month td.today { background-color:dodgerblue; } | + | |table.month td.today { background-color:dodgerblue; } |
table.month td { | table.month td { | ||
border: none; | border: none; | ||
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| - | {|style="font color= | + | {|style="font color="#ffffff"; "background-color:navy; cellpadding="0" cellspacing="4" border="4" bordercolor=dodgerblue; border-spacing:0px; text-align:center" width="250px" |
</table> | </table> | ||
{| align="center" | {| align="center" | ||
|-valign="top" | |-valign="top" | ||
| + | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=04}} | ||
|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=05}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=05}} | ||
|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=06}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=06}} | ||
|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=07}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=07}} | ||
| + | |- | ||
|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=08}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=08}} | ||
|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=09}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=09}} | ||
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|align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=11}} | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=11}} | ||
|} | |} | ||
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|} | |} | ||
Latest revision as of 07:46, 28 October 2008
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October 9, 2008Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them
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