Team:Hawaii/Notebook/2008-06- 4

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{{Team:Hawaii/Header}}
{{Team:Hawaii/Header}}
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= Things we did today =
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== Wetlab work ==
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=== Make Kan50 1000x Concentrate Stock ===
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:'''Krystle'''
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:* Nanopure H2O + Kanamycin
 +
:* stored in -20C freezer -> antibiotics box
-
== Make Kan50 1000x Concentrate Stock (KS) ==
+
===Make Amp50, Amp50+Kan20 LB Plates===
-
* Nanopure H2O + Kanamycin
+
:'''Krystle & Norman'''
-
* stored in -20C freezer -> antibiotics box
+
:* made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media here]
 +
:** x10 Amp 50ug/mL
 +
:** x10 Amp 50ug/mL + Kan 20ug/mL plates
 +
:* left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.
-
== Make Amp50, Amp50+Kan20 LB Plates (NW, KS) ==
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==Drylab Work==
 +
=== Signal Peptide Research  ===
 +
:'''Krystle'''
 +
:* found two papers with leader sequences {please post on CiteULike}
 +
:'''Norman'''
 +
:* blast sequence on Cu chealating protein, found PCC6803 gene and used [http://www.cbs.dtu.dk/services/SignalP/ SignalP 3.0] for signal peptide prediction
-
* made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media here]
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=== Discussion on CO<sub>2</sub> Incubator Controller ===
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** x10 Amp 50uL/mL
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:'''Dr. Gautz, Krystle & Norman'''
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** x10 Amp 50ul/mL + Kan 20uL/mL plates
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:* KS will do some fact finding about the parts required
-
* left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.
+
:* KS will put together schematic for CO2 regulator design
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== Help Krystle on her Signal Peptide Research (NW, KS) ==
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= Discussion =
-
* (KS) found two papers with leader sequences {please post on CiteULike}
+
-
* (NW) blast sequence on Cu chealating protein, found PCC6803 gene and used [http://www.cbs.dtu.dk/services/SignalP/ SignalP 3.0] for signal peptide prediction
+
-
== Discuss with Dr. Gautz on CO<sub>2</sub> Incubator Controller(KS, NW) ==
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= Quote of the Day =
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* KS will do some fact finding about the parts required
+
-
* KS will put together schematic for CO2 regulator design
+
-
 
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== Discussion ==
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{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}

Latest revision as of 03:28, 12 June 2008

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Contents

Things we did today

Wetlab work

Make Kan50 1000x Concentrate Stock

Krystle
  • Nanopure H2O + Kanamycin
  • stored in -20C freezer -> antibiotics box

Make Amp50, Amp50+Kan20 LB Plates

Krystle & Norman
  • made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media here]
    • x10 Amp 50ug/mL
    • x10 Amp 50ug/mL + Kan 20ug/mL plates
  • left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.

Drylab Work

Signal Peptide Research

Krystle
  • found two papers with leader sequences {please post on CiteULike}
Norman
  • blast sequence on Cu chealating protein, found PCC6803 gene and used [http://www.cbs.dtu.dk/services/SignalP/ SignalP 3.0] for signal peptide prediction

Discussion on CO2 Incubator Controller

Dr. Gautz, Krystle & Norman
  • KS will do some fact finding about the parts required
  • KS will put together schematic for CO2 regulator design

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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