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Team:Warsaw/Calendar-Main/11 June 2008
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| - | <h3> Blue/white and rifampicin test </h3><h4>Michał L., Ewa, | + | <h3> Blue/white and rifampicin test #1</h3><h4>Michał L., Ewa, Piotr</h4> |
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with: | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with: | ||
| - | <ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5 | + | <ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5</a>,</li> |
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a>,</li> | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a>,</li> | ||
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a>, </li> | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a>, </li> | ||
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul> | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul> | ||
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li> | <li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li> | ||
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| + | <li>Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara. </li> | ||
| + | </ol> | ||
</html> | </html> | ||
Latest revision as of 14:49, 26 October 2008
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Blue/white and rifampicin test #1Michał L., Ewa, Piotr
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