Team:Heidelberg/Notebook/Killing I/Cloning

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==Killing I - Cloning strategy==
==Killing I - Cloning strategy==
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In progress...
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Our goal to clone a modified bacteriophage λ which can be transported by conjugation, allows the selection of infected cells through a chloamphenicol resistance and harbours GFP for visualization was designed in two strategies whereas strategy two builds on strategy one. A detailed description of the two strategies can be seen below (see 1.1 and 1.2). The used λ cI protein generator was synthesised by geneart and just transferred in a standard plasmid as it can be seen in 1.3.
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=== phage cloning strategy one===
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[[Image:HD_phage1_klein.gif|900px]]
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=== phage cloning strategy two===
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[[Image:HD_phage2_klein.png|900px]]
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=== Bacteriophage λ cI cloning strategy===
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[[Image:HD_phage3.png|200px]]

Latest revision as of 09:08, 29 October 2008

Contents

Killing I - Cloning strategy

Our goal to clone a modified bacteriophage λ which can be transported by conjugation, allows the selection of infected cells through a chloamphenicol resistance and harbours GFP for visualization was designed in two strategies whereas strategy two builds on strategy one. A detailed description of the two strategies can be seen below (see 1.1 and 1.2). The used λ cI protein generator was synthesised by geneart and just transferred in a standard plasmid as it can be seen in 1.3.


phage cloning strategy one

HD phage1 klein.gif



phage cloning strategy two

HD phage2 klein.png


Bacteriophage λ cI cloning strategy

HD phage3.png

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