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Team:Chiba/Calendar-Home/17 August 2008
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| - | >[[Team:Chiba | + | >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]] |
| - | + | [[Team:Chiba/Calendar-Home/16 August 2008|16 August 2008 <]]|[[Team:Chiba/Calendar-Home/18 August 2008|> 18 August 2008]] | |
| - | [[Team:Chiba/Calendar-Home/16 August 2008|16 August 2008]] | + | |
==Laboratory work== | ==Laboratory work== | ||
| Line 11: | Line 10: | ||
:'''[[Team:Chiba/protocol/transformation|Transformation]]''' | :'''[[Team:Chiba/protocol/transformation|Transformation]]''' | ||
:competent cells : XL10G | :competent cells : XL10G | ||
| - | ::*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] --> No colonies on plates |
| - | ::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] --> No colonies on plates |
| - | ::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] --> No colonies on plates |
| - | ::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] --> No colonies on plates |
| - | ::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] --> No colonies on plates |
| - | ::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] -> No colonies on plates | + | ::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] --> No colonies on plates |
| - | + | ||
| - | + | ||
===Team:Output=== | ===Team:Output=== | ||
'''[[Team:Chiba/protocol/PCR|PCR]]''' | '''[[Team:Chiba/protocol/PCR|PCR]]''' | ||
| - | *[http://partsregistry.org/Part:BBa_E0430 BBa_E0430] | + | *[http://partsregistry.org/Part:BBa_E0430 BBa_E0430](1) |
| - | *[http://partsregistry.org/Part:BBa_E0420 BBa_E0420] | + | *[http://partsregistry.org/Part:BBa_E0420 BBa_E0420](2) |
| - | *[http://partsregistry.org/Part:BBa_J06702 BBa_J06702] | + | *[http://partsregistry.org/Part:BBa_J06702 BBa_J06702](3) |
| - | *[http://partsregistry.org/Part:BBa_S01416 BBa_S01416] | + | *[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](4) |
| - | * | + | *[http://partsregistry.org/Part:BBa_I730003 BBa_I730003](5) |
| - | * | + | *[http://partsregistry.org/Part:BBa_I730004 BBa_I730004](6) |
| - | * | + | *[http://partsregistry.org/Part:BBa_I763026 BBa_I763026](7) |
| - | *[http://partsregistry.org/Part:BBa_I763002 BBa_I763002] | + | *[http://partsregistry.org/Part:BBa_I763002 BBa_I763002](8) |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>DNA | + | <td>DNA template(μL)</td> |
| - | <td>1</td><td>1</td><td>1</td><td>1</td | + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>FW primer</td> | + | <td>FW primer(μL)</td> |
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>VR primer</td> | + | <td>VR primer(μL)</td> |
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dNTPmix</td> | + | <td>dNTPmix(μL)</td> |
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>thermo pol buffer</td> | + | <td>thermo pol buffer(μL)</td> |
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>DNA pol(VENT)</td> | + | <td>DNA pol(VENT)(μL)</td> |
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 05:11, 30 October 2008
16 August 2008 <|> 18 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- competent cells : XL10G
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] --> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] --> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] --> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] --> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] --> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] --> No colonies on plates
Team:Output
- [http://partsregistry.org/Part:BBa_E0430 BBa_E0430](1)
- [http://partsregistry.org/Part:BBa_E0420 BBa_E0420](2)
- [http://partsregistry.org/Part:BBa_J06702 BBa_J06702](3)
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](4)
- [http://partsregistry.org/Part:BBa_I730003 BBa_I730003](5)
- [http://partsregistry.org/Part:BBa_I730004 BBa_I730004](6)
- [http://partsregistry.org/Part:BBa_I763026 BBa_I763026](7)
- [http://partsregistry.org/Part:BBa_I763002 BBa_I763002](8)
| Sample No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| DNA template(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| FW primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| VR primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| dNTPmix(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT)(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O(μL) | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
| TOTAL(μL) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
