Team:Chiba/Calendar-Home/18 August 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 05:13, 30 October 2008

>Home | Notebook

17 August 2008 <|> 19 August 2008

Laboratory work

Team:Input

Transformation

[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

competent cells : XL10Gold(Cm),LB-agar(Kan^R)

Team:communication

PCR

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008)
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008)
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control

DNA Template(μL)	1
dNTP mix(μL)	10
Foward Primer(μL)	5
Reverse Primer(μL)	5
DNA polymerase(μL)	1
Thermopol Buffer(μL)	10
dH₂O(μL)	68
TOTAL(μL)	100

95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

--->Gel Check

Sample DNA	3
Loading Dye(μL)	2
dH₂O(μL)	7
TOTAL(μL)	12

From left;

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008) ->None
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) ->None
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) ->None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) ->None
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008) ->None
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK

Transformation

competent cells : XL10G

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2006) -> No colonies on plates(19/8)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

-->(20/8)Mini prep

Team:Output

no work

@@ Line 1: / Line 1: @@
 >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
 [[Team:Chiba/Calendar-Home/17 August 2008|17 August 2008 <]]|[[Team:Chiba/Calendar-Home/19 August 2008|> 19 August 2008]]
@@ Line 6: / Line 5: @@
 ==Laboratory work==
 ===Team:Input===
-パーツ起こし
-*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
-*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)
 '''[[Team:Chiba/protocol/transformation|Transformation]]'''
@@ Line 18: / Line 13: @@
 ===Team:communication===
 :'''[[Team:Chiba/protocol/PCR|PCR]]'''
-::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)]
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008)
-::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)]
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008)
-::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)]
+::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008)
-::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)]
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008)
-::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)]
+::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008)
-::*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620(2007)]･･･control
+::*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control
 <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">DNA Template</td>
+<td width="257">DNA Template(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dNTP mix</td>
+<td>dNTP mix(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Foward Primer</td>
+<td>Foward Primer(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Reverse Primer</td>
+<td>Reverse Primer(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>DNA polymerase</td>
+<td>DNA polymerase(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Thermopol Buffer</td>
+<td>Thermopol Buffer(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>68</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>100μL</td>
+<td>100</td>
 </tr>
 </table>
-:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+:95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C
@@ Line 78: / Line 73: @@
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>7</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>12μL</td>
+<td>12</td>
 </tr>
 </table>