Team:Warsaw/Calendar-Main/10 September 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>,<h3><h3/><h4> Emila</h4>
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<h3>Tests for ampicillin resistance given by protein added to medium</h3>
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<h4>Piotr, Emila</h4>
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<ol><li>Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
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<li><p>Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> to LB + 0.25 mM IPTG and kanamycin.</li>
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<ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
 
<li>Measurement of growth after 6 hours.</li></ol>
<li>Measurement of growth after 6 hours.</li></ol>
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<table id="result">
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<tr><th rowspan="2">Prey: Z-omega </th><th>amount in picomoles per mL</th><th> 4,920</th><th> 12,301</th><th> 24,602</th><th> 61,504</th><th>123,009 </th><th>246,018</th><th> 369,026</th></tr>
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<tr><th>OD </th><th>0 </th><th>0,21 </th><th>0,34</th><th> 0,62 </th><th>0,89 </th><th>0,91 </th><th>0,92</th></tr>
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<tr><th rowspan="2">Prey: Z-alpha </th><th>amount in picomoles per mL</th><th>4,333</th><th> 8,692</th><th> 17,383</th><th> 43,485</th><th> 86,996</th><th> 173,991</th><th>260,987</th></tr>
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<tr><th> OD </th><th>0</th><th> 0,25</th><th> 0,42</th><th> 0,71</th><th> 0,9</th><th> 0,95</th><th> 0,98</th></tr>
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<tr><th rowspan="2">Prey: A-alpha</th><th> amount in picomoles per mL</th><th> 5,600 </th><th>11,234</th><th> 22,468 </th><th>56,204</th><th>112,443</th><th> 224,885</th><th> 337,328</th></tr>
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<tr><th> OD </th><th>0 </th><th>0,19 </th><th>0,38 </th><th>0,65</th><th> 0,85 </th><th>1,00 </th><th>1,03</th></tr>
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<img src="https://static.igem.org/mediawiki/2008/6/65/Wykres-piotr.jpg">
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<var>Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da. </var>
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Tabela z wynikami z exela- minimalna ilość biąłka do porzywki (ilość bilałka dodaną do porztwki przeliczono na nanomole białka- tu dać ile lażde z białek ma masy molowej):
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<p>Z_omega was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a><br>
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Z_alpha was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>.<br>
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A_alpha was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>.</p>
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Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

  1. Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).

  2. Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.

  3. Measurement of growth after 6 hours.


Prey: Z-omega amount in picomoles per mL 4,920 12,301 24,602 61,504123,009 246,018 369,026
OD 0 0,21 0,34 0,62 0,89 0,91 0,92
Prey: Z-alpha amount in picomoles per mL4,333 8,692 17,383 43,485 86,996 173,991260,987
OD 0 0,25 0,42 0,71 0,9 0,95 0,98
Prey: A-alpha amount in picomoles per mL 5,600 11,234 22,468 56,204112,443 224,885 337,328
OD 0 0,19 0,38 0,65 0,85 1,00 1,03
Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.

Z_omega was added to bacterial culture expressing pACYC177+OmpA_A_alpha
Z_alpha was added to bacterial culture expressing pACYC177+OmpA_A_omega.
A_alpha was added to bacterial culture expressing pACYC177+OmpA_Z_omega.

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