Team:Warsaw/Calendar-Main/10 September 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 21:12, 26 October 2008


	Attribution analyzer / Interactive list of topics

Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).
Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.
Measurement of growth after 6 hours.

Prey: Z-omega	amount in picomoles per mL	4,920	12,301	24,602	61,504	123,009	246,018	369,026
Prey: Z-omega	OD	0	0,21	0,34	0,62	0,89	0,91	0,92
Prey: Z-alpha	amount in picomoles per mL	4,333	8,692	17,383	43,485	86,996	173,991	260,987
Prey: Z-alpha	OD	0	0,25	0,42	0,71	0,9	0,95	0,98
Prey: A-alpha	amount in picomoles per mL	5,600	11,234	22,468	56,204	112,443	224,885	337,328
Prey: A-alpha	OD	0	0,19	0,38	0,65	0,85	1,00	1,03

Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.

Z_omega was added to bacterial culture expressing pACYC177+OmpA_A_alpha
Z_alpha was added to bacterial culture expressing pACYC177+OmpA_A_omega.
A_alpha was added to bacterial culture expressing pACYC177+OmpA_Z_omega.

@@ Line 8: / Line 8: @@
 <h4>Piotr, Emila</h4>
-<ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
+<ol><li>Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
-<li><p>Inoculation of OmpA_A_alfa, Omp_A_omega, Omp_Z_omega, Omp_alfa and Omp_omega to LB + 0.25 mM IPTG and kanamycin.</li>
+<li><p>Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> to LB + 0.25 mM IPTG and kanamycin.</li>
 <li>Measurement of growth after 6 hours.</li></ol>
+<br><br>
+<table id="result">
+<tr><th rowspan="2">Prey: Z-omega	</th><th>amount in picomoles per mL</th><th>	4,920</th><th>	12,301</th><th>	24,602</th><th>	61,504</th><th>123,009	</th><th>246,018</th><th>	369,026</th></tr>
+<tr><th>OD	</th><th>0	</th><th>0,21	</th><th>0,34</th><th>	0,62	</th><th>0,89	</th><th>0,91	</th><th>0,92</th></tr>
-Tabela z wynikami z exela- minimalna ilość biąłka do porzywki (ilość bilałka dodaną do porztwki przeliczono na nanomole białka- tu dać ile lażde z białek ma masy molowej):
+<tr><th rowspan="2">Prey: Z-alpha	</th><th>amount in picomoles per mL</th><th>4,333</th><th>	8,692</th><th>	17,383</th><th>	43,485</th><th>	86,996</th><th>	173,991</th><th>260,987</th></tr>
+<tr><th>	OD	</th><th>0</th><th>	0,25</th><th>	0,42</th><th>	0,71</th><th>	0,9</th><th>	0,95</th><th>	0,98</th></tr>
+<tr><th rowspan="2">Prey: A-alpha</th><th>	amount in picomoles per mL</th><th>	5,600	</th><th>11,234</th><th>	22,468	</th><th>56,204</th><th>112,443</th><th>	224,885</th><th>	337,328</th></tr>
+<tr><th>	OD	</th><th>0	</th><th>0,19	</th><th>0,38	</th><th>0,65</th><th>	0,85	</th><th>1,00	</th><th>1,03</th></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2008/6/65/Wykres-piotr.jpg">
+<var>Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da. </var>
+<p>Z_omega was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a><br>
+Z_alpha was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>.<br>
+A_alpha was added to bacterial culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>.</p>
 </html>