Team:Chiba/Calendar-Home/31 August 2008

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(Difference between revisions)

Latest revision as of 20:41, 29 October 2008

>Home | Notebook

30 August 2008 <|> 1 September 2008

Laboratory work

Team:Input

no work

Team:Communication

Transformation

Competent Cells : XL10Gold

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)

---> We saved these plates with a refrigerator.

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3	4
Sample DNA(μL)	12	10
PstⅠ(μL)	0.2	0.2
XbaⅠ(μL)	0.2	-
SpeⅠ(μL)	-	0.4
Buffer 2(μL)	-	1.5
Buffer 3(μL)	2	-
BSA(μL)	2	1.5
dH₂O(μL)	3.6	1.4
TOTAL(μL)	20	15

--->(1/9)Gel Check

(30/8)--->Mini prep

--->Digestion test

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Sample	Single Digesiton 2~5	Double Digestion 2~5	Single Digestion T9002(1)	Single Digesiton T9002(2)	Double Digestion T9002(1)(2)
Sample DNA(μL)	1	3	3	1	3
XbaI(μL)	0.1	0.1	0.1	0.1	0.1
SpeI(μL)	-	0.1	-	-	0.1
Buffer 2(μL)	0.9	0.8	0.9	0.9	0.8
BSA(μL)	1	1	1	1	1
dH₂O(μL)	7	5	5	7	5
TOTAL(μL)	10	10	10	10	10

--->Gel Check

Sample No.	[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2)	Single & Double Digestion
Sample DNA(μL)	1	10
Loading Dye(μL)	1	2
dH₂O(μL)	4	-
TOTAL(μL)	6	12

From Left

Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Team:Output

Digestion

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)

Sample No.	1	2
DNA	10	10
SpeI	1	0
PstI	1	1
XbaI	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50μl	50μl

-->left for 3 hours at 37 °C.

-->(1)20μl refine

-->(2)20μl refine

@@ Line 70: / Line 70: @@
 :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 ::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
-::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
 <table width="600" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample</td>
-<td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002①</td><td>Single Digesiton T9002②</td><td>Double Digestion T9002①②</td>
+<td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002(1)</td><td>Single Digesiton T9002(2)</td><td>Double Digestion T9002(1)(2)</td>
 </tr>
 <tr>
@@ Line 81: / Line 81: @@
 </tr>
 <tr>
-<td>XbaⅠ(μL)</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+<td>XbaI(μL)</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>SpeⅠ(μL)</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
+<td>SpeI(μL)</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
 </tr>
 <tr>
@@ Line 113: / Line 113: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②
+<td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2)
 </td><td>Single & Double Digestion</td>
 </tr>
@@ Line 134: / Line 134: @@
 :From Left
 ::*Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
-::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
 ::*Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
-::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
-::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
 |}
 ===Team:Output===
 [[Team:Chiba/protocol/digestion|Digestion]]
-*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
-*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td>
+<td>1</td><td>2</td>
 </tr>
 <tr>
@@ Line 159: / Line 156: @@
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI</td>
 <td>1</td><td>0</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaI</td>
 <td>0</td><td>1</td>
 </tr>
@@ Line 192: / Line 189: @@
 </table>
--->left for 3 hours at 37 degrees.
+-->left for 3 hours at 37 °C.
--->①20μl refine
+-->(1)20μl refine
--->②20μl refine
+-->(2)20μl refine