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Team:Warsaw/Calendar-Main/1 July 2008
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li> | ||
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li> | ||
| - | <li>Gel electrophoresis of digested DNA - there where no proper plasmids</li> | + | <li>Gel electrophoresis of digested DNA - there where no proper plasmids.</li> |
| - | <li>Isolation of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a></li> | + | <li>Isolation of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>.</li> |
| - | <li>Isolation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>( | + | <li>Isolation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator).</li> |
| - | <li>Overnight digest of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>( | + | <li>Overnight digest of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator) with NotI.</li> |
<li> | <li> | ||
| - | Overnight digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with NotI and <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing>dephosphorylation</a> with CIAP | + | Overnight digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with NotI and <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing>dephosphorylation</a> with CIAP. |
</li></ol> | </li></ol> | ||
| - | <h3>Cloning | + | <h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> |
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
<ol> | <ol> | ||
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| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3><h4>Paweł</h4> |
<p><ol> | <p><ol> | ||
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid.</li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid.</li> | ||
Latest revision as of 14:04, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, Marcin
We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct. Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPaweł
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