"
Brown: Team Resistance/25 October 2008
From 2008.igem.org
(New page: ==25 October 2008== ====Conductance/Lysis Re-test==== Same method as oct 22 but used 50 mL of same culture as control and test For some reason, the conductivity of the control solution ...) |
(→Conductance/Lysis Re-test) |
||
| (7 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{BNBK}} | ||
| + | |||
| + | |||
==25 October 2008== | ==25 October 2008== | ||
| Line 7: | Line 10: | ||
For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters | For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters | ||
| + | At T=180min, added 600ul more arabinose solution, to make culture with 2% arabinose 2% 50mL solution = 1.04 mL | ||
| + | At T=205, added 1mL more arabinose to actually make it 2% solution | ||
| + | Cells did not lyse today. Perhaps wrong plasmid used. Will make culture of s different glycerol stock for test tomorrow. | ||
| + | |||
| + | For purposes of current method, 50mL of culture is not needed for each test. | ||
| + | |||
| + | Will test 15mL of each culture tomorrow (total 30mL)and perhaps arabinose induction will be more efficient. | ||
====pVJ4 biobrick==== | ====pVJ4 biobrick==== | ||
| Line 14: | Line 24: | ||
Ran gel of part: | Ran gel of part: | ||
| + | |||
| + | *Single band between 3 and 4kb | ||
| + | |||
| + | EcoRI/SpeI digest | ||
| + | *Used Buffer B due to potential star activity with EcoRI | ||
| + | |||
| + | ====Conductance/Lysis Re-test==== | ||
| + | |||
| + | *Same method as oct 22 but used 50 mL of same culture as control and test | ||
| + | |||
| + | |||
| + | *Time control Test | ||
| + | OD OD | ||
| + | *0 0.064 0.063 | ||
| + | *15 0.061 0.061 | ||
| + | *30 0.072 0.065 | ||
| + | *45 0.074 0.072 | ||
| + | *60 0.077 0.074 | ||
| + | *75 0.081 0.078 | ||
| + | *90 0.085 0.084 | ||
| + | *105 0.089 0.088 | ||
| + | *120 0.093 0.086 | ||
| + | *135 0.093 0.092 | ||
| + | *150 0.088 0.087 | ||
| + | *165 0.086 0.09 | ||
| + | *180 0.092 0.09 | ||
| + | *195 0.089 0.083 | ||
| + | *210 0.091 0.089 | ||
| + | *225 0.095 0.088 | ||
| + | *240 0.089 0.089 | ||
| + | *255 0.09 0.083 | ||
| + | *270 0.087 0.086 | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | *For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters | ||
Latest revision as of 11:34, 27 October 2008
|
25 October 2008Conductance/Lysis Re-testSame method as oct 22 but used 50 mL of same culture as control and test For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters At T=180min, added 600ul more arabinose solution, to make culture with 2% arabinose 2% 50mL solution = 1.04 mL At T=205, added 1mL more arabinose to actually make it 2% solution Cells did not lyse today. Perhaps wrong plasmid used. Will make culture of s different glycerol stock for test tomorrow. For purposes of current method, 50mL of culture is not needed for each test. Will test 15mL of each culture tomorrow (total 30mL)and perhaps arabinose induction will be more efficient. pVJ4 biobrickconcentration miniprep: 63.6ng/ul Ran gel of part:
EcoRI/SpeI digest
Conductance/Lysis Re-test
OD OD *0 0.064 0.063 *15 0.061 0.061 *30 0.072 0.065 *45 0.074 0.072 *60 0.077 0.074 *75 0.081 0.078 *90 0.085 0.084 *105 0.089 0.088 *120 0.093 0.086 *135 0.093 0.092 *150 0.088 0.087 *165 0.086 0.09 *180 0.092 0.09 *195 0.089 0.083 *210 0.091 0.089 *225 0.095 0.088 *240 0.089 0.089 *255 0.09 0.083 *270 0.087 0.086
|
