"
Team:Hawaii/Parts
From 2008.igem.org
(Difference between revisions)
(→Parts in process (building)) |
|||
| (6 intermediate revisions not shown) | |||
| Line 30: | Line 30: | ||
==Parts in process (building)== | ==Parts in process (building)== | ||
| + | {| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center" | ||
| + | |- | ||
| + | ! width="75"|Part number | ||
| + | ! width="250"|Description | ||
| + | ! width="75"|Type | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010'''] | ||
| + | | Broad-Host-Range plasmid derived from RSF1010 | ||
| + | | plasmid | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 '''K125330'''] | ||
| + | | P1 lytic replication | ||
| + | | | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 '''K125340'''] | ||
| + | | oriV | ||
| + | | | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 '''K125510'''] | ||
| + | | Plasmid replication proteins | ||
| + | | protein coding | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520'''] | ||
| + | | aadA gene | ||
| + | | protein coding | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800'''] | ||
| + | | rep proteins with promoter and rbs | ||
| + | | protein coding | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 '''K125830'''] | ||
| + | | ''Synechocystis''-specific, nitrate-inducible GFP secretion device under the control of a ''nirA'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) | ||
| + | | device | ||
| + | |} | ||
| + | |||
| + | ==Parts submitted== | ||
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center" | {| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center" | ||
|- | |- | ||
| Line 37: | Line 73: | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000'''] | ||
| - | | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 ( | + | | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (''lac'' promoter+''lacZ'') |
| plasmid | | plasmid | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| Line 46: | Line 82: | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110'''] | ||
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] | | nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] | ||
| - | |||
| - | |||
| - | |||
| - | |||
| regulator | | regulator | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| Line 64: | Line 96: | ||
| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| - | | [http://partsregistry.org/wiki/index.php/Part: | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810'''] |
| - | | N-terminal slr2016 signal sequence attached to GFP fusion protein | + | | N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) |
| + | | protein coding | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820'''] | ||
| + | | ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) | ||
| + | | device | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350'''] | ||
| + | | oriTr/LacZ | ||
| + | | conjugation screening | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360'''] | ||
| + | | oriT/LacZ | ||
| + | | conjugation screening | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320'''] | ||
| + | | oriT derived from RP4 | ||
| + | | origin of transfer | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020'''] | ||
| + | | rep/oriv/aada/orit | ||
| + | | intermediate for RSF1010 derived plasmid | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530'''] | ||
| + | | aadA gene with promoter and rbs | ||
| protein coding | | protein coding | ||
|} | |} | ||
| - | |||
| - | |||
Latest revision as of 03:53, 30 October 2008
Contents |
Guidelines for naming parts
| Part number | Part type |
|---|---|
| K125000-K125099 | plasmid |
| K125100-K125199 | promoter |
| K125200-K125299 | ribosome binding site |
| K125300-K125499 | signal sequences, conjugation sequences, random stuff |
| K125500-K125699 | protein coding |
| K125700-K125799 | transcription terminator |
| K125800-K125999 | devices |
Parts planned
Parts in process (building)
| Part number | Description | Type |
|---|---|---|
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] | Broad-Host-Range plasmid derived from RSF1010 | plasmid |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 K125330] | P1 lytic replication | |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 K125340] | oriV | |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 K125510] | Plasmid replication proteins | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] | aadA gene | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] | rep proteins with promoter and rbs | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 K125830] | Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) | device |
Parts submitted
| Part number | Description | Type |
|---|---|---|
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) | plasmid |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] | nitrate inducible nir promoter | regulator |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] | nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] | regulator |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] | cyanobacterial pilA derived amino termninus protein secretion signal sequence | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] | cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] | GFP fusion protein | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] | N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) | protein coding |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] | Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) | device |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] | oriTr/LacZ | conjugation screening |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] | oriT/LacZ | conjugation screening |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] | oriT derived from RP4 | origin of transfer |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] | rep/oriv/aada/orit | intermediate for RSF1010 derived plasmid |
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] | aadA gene with promoter and rbs | protein coding |
