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Team:Hawaii/Notebook/2008-10-26
From 2008.igem.org
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::* ~2ng/μl | ::* ~2ng/μl | ||
::* The prep kit yields really clean preps in ~1 hour. :D! | ::* The prep kit yields really clean preps in ~1 hour. :D! | ||
| + | |||
| + | ===Plasmid prep=== | ||
| + | [[Image:102508plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.]] | ||
| + | :<strong> Margaret</strong> | ||
| + | |||
| + | ===Construction of BB-pRL1383a/K125820=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Transformation of overnight ligation products | ||
| + | :* Plated onto LB sp<sub>100</sub> plates | ||
| + | |||
| + | ==Drylab Work== | ||
| + | ===Inputted parts in the registry=== | ||
| + | :<strong>Krystle</strong> | ||
= Discussion = | = Discussion = | ||
Latest revision as of 03:41, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
- Ran on gel to verify plasmid
- ~2ng/μl
- The prep kit yields really clean preps in ~1 hour. :D!
Plasmid prep
- Margaret
Construction of BB-pRL1383a/K125820
- Krystle
- Transformation of overnight ligation products
- Plated onto LB sp100 plates
Drylab Work
Inputted parts in the registry
- Krystle
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
