Latest revision as of 17:26, 19 June 2008

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Things we did today

Wetlab work

Test Transform pRL1383a Plasmids from yesterday

KS

cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify

Counted transformation efficiency of new competent cells

Grace

**Colony Count of Transformants**
Plasmid	Dilution	Colony Forming Units on Plate	Number of Transformed Cells per mL
pUC18	1:1	79	3950
	1:10	12	6000
	1:100	1	5000
BBa_B0024	1:1	0	n/a
BBa_B0025	1:1	1	50
BBa_E0240	1:1	0	n/a

Discussion

Even though our cells appear more competent than the last batch, we still have a long way to go compared to the commercial grade competent cells. It would be a better idea to chill the tubes, aliquot, then ethanol freeze them.

Quote of the Day

"What's that band-aid for on your hand"-NW

"I was testing out this bottle of anesthetics then pricked myself with needles"-KS

"Umm OK..."-NW

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 6: / Line 6: @@
 :'''KS'''
 ''cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify''
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
-== Drylab Work ==
-===Name of Task===
-:<strong> name of person/people who performed the task</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. read through papers, worked on proposal, etc.
+=== Counted transformation efficiency of new competent cells===
+: '''Grace'''
+{| border="1"
+|+ '''Colony Count of Transformants'''
+!Plasmid
+!Dilution
+!Colony Forming Units on Plate
+!Number of Transformed Cells per mL
+|-
+| rowspan="3" align="center"|pUC18||align="center"| 1:1 || align="center"|79|| align="center"|3950
+|-
+|  align="center"|1:10|| align="center"|12|| align="center"|6000
+|-
+|  align="center"|1:100|| align="center"|1||align="center"|5000
+|-
+| " align="center"|BBa_B0024||align="center"|1:1||align="center"|0||align="center"|n/a
+|-
+| align="center"|BBa_B0025||align="center"|1:1|| align="center"|1|| align="center"|50
+|-
+| align="center"|BBa_E0240||align="center"|1:1|| align="center"|0 ||align="center"|n/a
+|}
 = Discussion =
+Even though our cells appear more competent than the last batch, we still have a long way to go compared to the commercial grade competent cells. It would be a better idea to chill the tubes, aliquot, ''then'' ethanol freeze them.
 = Quote of the Day =

Team:Hawaii/Notebook/2008-06-18

From 2008.igem.org