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Alberta NINT/16 May 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/15_May_2008 | < Previous entry]] | + | [[Alberta_NINT/15_May_2008 | < Previous entry]] | [[Alberta_NINT/20_May_2008 | Next entry >]] |
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lab work (SD, JD): | lab work (SD, JD): | ||
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insertion into plasmid. | insertion into plasmid. | ||
Ran on 2% agarose gel. | Ran on 2% agarose gel. | ||
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Latest revision as of 21:40, 11 October 2008
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lab work (SD, JD):
PCR of T/A I oligonucleotide using Term + and Term - primers
DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
insertion into plasmid.
Ran on 2% agarose gel.
