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Alberta NINT/20 June 2008
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(Difference between revisions)
(New page: lab work (SD): Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. Incubated at 37 C for 2 hrs. Ran K102002.1-.6 on 2% agarose gel at 1...) |
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lab work (SD): | lab work (SD): | ||
Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. | Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. | ||
| Line 6: | Line 11: | ||
Quantified DNA for K102002 and TA0. | Quantified DNA for K102002 and TA0. | ||
Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min. | Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min. | ||
| + | [[Image:NINT_SD_p46.jpg|750 px]] | ||
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| + | lab work (JD): | ||
| + | Digested K102005 with BamH1 + NcoI and incubated for 2 hrs at 37C | ||
| + | Ran on 2% agarose gel slowly at 90V | ||
| + | Cut out desired fragment and gel purified | ||
Latest revision as of 22:52, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI.
Incubated at 37 C for 2 hrs.
Ran K102002.1-.6 on 2% agarose gel at 110V for 50 min.
Purified TA0 using QIAquick nucleotide removal protocol.
Quantified DNA for K102002 and TA0.
Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min.
lab work (JD):
Digested K102005 with BamH1 + NcoI and incubated for 2 hrs at 37C
Ran on 2% agarose gel slowly at 90V
Cut out desired fragment and gel purified
