Team:BrownTwo/Limiter/altdesign

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Latest revision as of 06:05, 30 October 2008

Examples of Additional Control Systems

The synthetic transcriptional control system that we have added to the registry is a powerful tool, as its elements could be put together in an enormous variety of ways. These are some possibilities that we have drawn up:

up-limiter - upregulates a gene when the expression falls below a certain level
middle-manager- a combination limiter and up-limiter, it keeps a gene's expression between two threshold levels
dual limiter - regulates two genes simultaneously based on the induction of one.
limiter device as a regulatory bridge between two pathways- the induction of one gene causes threshold regulation of another
directed evolution for transcriptional phenotypes - express a cell-death gene if the level of induction a given gene is above or below a threshold level

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 {{limitertemp}}
-Alternative construction and design possiblities
+==Examples of Additional Control Systems==
-*Smolke’s gal2 knockout scheme for using the GAL1 promoter
-*Finding additional inducible/repressible promoters to use in the system
-*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
-*alternatively, could consider having the promoter for construct Y produce an shRNA instead of a repressor protein
-*need to consider the possibility for regulation on both levels of protein synthesis
-Possible further modifications to these transcription factors include the use of different regulation domains,
+The synthetic transcriptional control system that we have added to the registry is a powerful tool, as its elements could be put together in an enormous variety of ways.  These are some possibilities that we have drawn up:
-Involves the consideration of multiple binding sites
+*up-limiter - upregulates a gene when the expression falls below a certain level
+*middle-manager- a combination limiter and up-limiter, it keeps a gene's expression between two threshold levels
+*dual limiter - regulates two genes simultaneously based on the induction of one.
+*limiter device as a regulatory bridge between two pathways- the induction of one gene causes threshold regulation of another
+*directed evolution for transcriptional phenotypes - express a cell-death gene if the level of induction a given gene is above or below a threshold level