Team:LCG-UNAM-Mexico/Experiments

From 2008.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 89: Line 89:
         <table border="0" cellspacing="0" cellpadding="2" width="590">
         <table border="0" cellspacing="0" cellpadding="2" width="590">
<p align="center">
<p align="center">
-
   <img src="https://static.igem.org/mediawiki/2008/1/19/Boton_exp1.jpg" width="190" height="31" />
+
   <a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments"><img src="https://static.igem.org/mediawiki/2008/1/19/Boton_exp1.jpg"  border="0" width="190" height="31" /></a>
-
<img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" width="190" height="31" /><img src="https://static.igem.org/mediawiki/2008/f/fc/Boton_exp3.jpg" width="190" height="31" /></p>
+
<!--<a href="#design"><img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" border="0" width="190" height="31" /></a> -->
-
<p align="center"><img src="https://static.igem.org/mediawiki/2008/a/ad/Boton_exp4.jpg" width="190" height="31" /></p>
+
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Design"><img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" border="0" width="190" height="31" /></a>
 +
 
 +
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Experiments"> <img src="https://static.igem.org/mediawiki/2008/f/fc/Boton_exp3.jpg" border="0" width="190" height="31" /></a></p>
 +
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Results"><p align="center"><img src="https://static.igem.org/mediawiki/2008/a/ad/Boton_exp4.jpg" border="0" width="190" height="31" /> </a></p>
  </p>
  </p>
<img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" width="579" height="9" />
<img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" width="579" height="9" />
             <tr>
             <tr>
-
           <td class="pageName"><div align="center">Wet Lab</div>            </td>
+
           <td class="pageName"><div align="center"><br>
 +
            Wet Lab</div>            </td>
         </tr>
         </tr>
         <tr>
         <tr>
Line 101: Line 105:
     </table>
     </table>
         <p align="justify"></p>
         <p align="justify"></p>
-
         <br>
+
          
-
        <br>
+
         <p align="justify" class="bodyText">The main goal of the experimental work is to test our system's performance. We expect to achieve this by assembling two devices which will carry the system components. Once we have them we will proceed to test the system's behavior.       </p>
-
         <p align="justify">&nbsp; The main goal of the experimental work is to test our system's performance. We expect to achieve this by assembling two devices which will carry the system components. Once we have them we will proceed to test the system's behavior. </p>
+
         <p align="justify" class="bodyText">The first step is to assemble both devices. One of them includes <em>luxR</em>, <em>c</em>I+LVA and<em> aiiA</em>; the other one carries the cI promoter, RcnR operator and the <em>rcnA</em> gene. We will use conventional techniques, to assemble all parts and transform the final receptor cell. The final plasmids will be pRK415 and pBBR1MCS-5 respectively.</p>
-
         <p align="justify">&nbsp; The first step is to assemble both devices. One of them includes LuxR, cI+ALV and aiiA; the other one carries the cI promoter, RcnR operator and the RcnA gene. We will use conventional techniques, to assemble all parts and transform the final receptor cell. The final plasmids will be pRK415 and pBBR1MCS-5 respectively.</p>
+
         <p align="justify"><span class="bodyText"> As a second step, we will proceed to test our system's behavior. Once we get YohM- cells (YohM was the name that the RcnA ORF used to have) with the RcnA device (BBa_K119009), we will measure changes in the medium's resistivity in order to get the nickel's extrusion and internalization parameters.  Our second device (BBa_K119010/BBa_K119011), will let us control our system. We expect to manipulate the concentration of nickel in the medium, which will be shown through the measurements and the sound emision. </span><br />
-
         <p align="justify">&nbsp; As a second step, we will proceed to test our system's behavior. Once we get YohM- cells(YohM was the name that the ORF coding RcnA used to have) with the RcnA device (BBa_K119009), we will measure changes in the medium's resistivity in order to get the nickel's extrusion and internalization parameters.  Our second device (BBa_K119010/BBa_K119011), will let us control our system. We expect to be manipulating the Nickel's concentration in the medium which will be shown through the measurements and the sound emision. <br />
+
      </p>
-
        </p>
+
         <div align="center">
-
         <div align="center"><br>
+
          <p><br>
-
          <br>       
+
            <br>       
-
          <img src="https://static.igem.org/mediawiki/2008/e/e5/Plasmid_LuxR.png" border="0" />
+
            <img src="https://static.igem.org/mediawiki/2008/e/e5/Plasmid_LuxR.png" border="0" />
-
          <img src="https://static.igem.org/mediawiki/2008/6/6a/Plasmid_Rcna.png" border="0" />
+
            <img src="https://static.igem.org/mediawiki/2008/6/6a/Plasmid_Rcna.png" border="0" /></p>
 +
          <p>&nbsp;</p>
 +
          <p align="center">
            
            
-
          <br>
+
          <br>
-
          <br>
+
            <br>
-
         
+
           
-
              <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /></p>
+
            <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" />         </p>
-
             </div>
+
              
 +
          </p>
 +
          <p align="center">
 +
  <a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments"><img src="https://static.igem.org/mediawiki/2008/c/cd/Boton_back.jpg"  border="0" width="190" height="31" /></a>
 +
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Design"><img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" border="0" width="190" height="31" /></a>
 +
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Experiments"> <img src="https://static.igem.org/mediawiki/2008/f/fc/Boton_exp3.jpg" border="0" width="190" height="31" /></a></p>
 +
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Results"><p align="center"><img src="https://static.igem.org/mediawiki/2008/a/ad/Boton_exp4.jpg" border="0" width="190" height="31" /></p>
 +
</p>
 +
      </div>
     <td width="132">&nbsp;</td>
     <td width="132">&nbsp;</td>
   </tr>
   </tr>

Latest revision as of 03:18, 30 October 2008

LCG-UNAM-Mexico:Experiments

Header image
iGEM 2008 TEAM
line decor
  
line decor

 
 
 
 



Wet Lab

The main goal of the experimental work is to test our system's performance. We expect to achieve this by assembling two devices which will carry the system components. Once we have them we will proceed to test the system's behavior.

The first step is to assemble both devices. One of them includes luxR, cI+LVA and aiiA; the other one carries the cI promoter, RcnR operator and the rcnA gene. We will use conventional techniques, to assemble all parts and transform the final receptor cell. The final plasmids will be pRK415 and pBBR1MCS-5 respectively.

As a second step, we will proceed to test our system's behavior. Once we get YohM- cells (YohM was the name that the RcnA ORF used to have) with the RcnA device (BBa_K119009), we will measure changes in the medium's resistivity in order to get the nickel's extrusion and internalization parameters. Our second device (BBa_K119010/BBa_K119011), will let us control our system. We expect to manipulate the concentration of nickel in the medium, which will be shown through the measurements and the sound emision.



 



ribbon