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Wisconsin:Lignin Project/28 August 2008
From 2008.igem.org
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Performed new sequencing PCR reaction.<br> | Performed new sequencing PCR reaction.<br> | ||
srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.<br> | srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.<br> | ||
| - | Digestion was verified on a gel and purified through gel extraction. | + | Digestion was verified on a gel and purified through gel extraction. <br> |
| + | Overnight ligation of insert into pSB1AK3 was allowed to run overnight.<br> | ||
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| + | '''Team Fungus:'''<br> | ||
| + | Ran Azure B assay on 96 well plate.<br> | ||
| + | Ran SDS PAGE gel.<br> | ||
| + | Ran western blot with T7 tag antibody.<br> | ||
| + | Grew overnight cultures for 5.r1.23 and 5.r1.20 | ||
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Latest revision as of 09:50, 29 October 2008
| Team Sorbitol: New primers arrived for gap between srlA, and srlE. Team Fungus: |
