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Team:The University of Alberta/29 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: Winnie did digestion of ER 1 and ER 2 with Age/Ngo in Buffer 4 loaded on a 1% gel) |
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Winnie did digestion of ER 1 and ER 2 with Age/Ngo in Buffer 4 | Winnie did digestion of ER 1 and ER 2 with Age/Ngo in Buffer 4 | ||
loaded on a 1% gel | loaded on a 1% gel | ||
| + | |||
| + | James | ||
| + | ran PCR product of colony PCRs done on spetember 26 | ||
| + | gel 1 looks ok, gel too seems like to have some contamination | ||
| + | gel1 | ||
| + | lane 2-9 are colonies 1-8 from ligations of J61003 with(21+22+23) | ||
| + | lane 10-16 are colonies 1-7 from ligations of J61003 with (21+23) | ||
| + | correct size 3kb-1.7kb | ||
| + | need to do sequencing to confirm J6 is there | ||
| + | |||
| + | Gel2. | ||
| + | lane 1-8=colonies of ligation of J61003+(22+24) | ||
| + | lane9=1kb ladder | ||
| + | lane10-16 are colonies of ligations of J61003+(25+24) | ||
| + | looks like some contaimination was there | ||
Latest revision as of 02:24, 29 October 2008
Winnie did digestion of ER 1 and ER 2 with Age/Ngo in Buffer 4 loaded on a 1% gel
James ran PCR product of colony PCRs done on spetember 26 gel 1 looks ok, gel too seems like to have some contamination gel1 lane 2-9 are colonies 1-8 from ligations of J61003 with(21+22+23) lane 10-16 are colonies 1-7 from ligations of J61003 with (21+23) correct size 3kb-1.7kb need to do sequencing to confirm J6 is there
Gel2. lane 1-8=colonies of ligation of J61003+(22+24) lane9=1kb ladder lane10-16 are colonies of ligations of J61003+(25+24) looks like some contaimination was there
