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Team:The University of Alberta/3 October 2008
From 2008.igem.org
(Difference between revisions)
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**transformed tom's ligation from yesterday,incubate overnight in the 37degree | **transformed tom's ligation from yesterday,incubate overnight in the 37degree | ||
**ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight | **ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight | ||
| + | |||
| + | ==David== | ||
| + | i miniprepped yesterdays cultures and checked them with a NotI digest. | ||
| + | |||
| + | pTET+BisdA worked | ||
| + | pTET+BisdB worked | ||
Latest revision as of 03:39, 29 October 2008
- James
- transformed tom's ligation from yesterday,incubate overnight in the 37degree
- ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight
David
i miniprepped yesterdays cultures and checked them with a NotI digest.
pTET+BisdA worked pTET+BisdB worked
