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Team:The University of Alberta/21 October 2008
From 2008.igem.org
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==David== | ==David== | ||
| - | + | *digested: | |
pUC57-pTET-BisdA with SphI+PstI with CIP treatment | pUC57-pTET-BisdA with SphI+PstI with CIP treatment | ||
pTET-BisdB with XbaI+PstI | pTET-BisdB with XbaI+PstI | ||
laqIQ-ERE-TetR with XbaI+PstI | laqIQ-ERE-TetR with XbaI+PstI | ||
| - | + | * set up the following ligations with the gel purified products: | |
pUC57-pTET-BisdA+pTET-BisdB | pUC57-pTET-BisdA+pTET-BisdB | ||
pSB1A3+laqIQ-ERE-TetR | pSB1A3+laqIQ-ERE-TetR | ||
| - | overnight at 16 degrees C. | + | * overnight at 16 degrees C. |
| + | ===TOm=== | ||
| + | *sequenced ETFA/B col5. with reverse universal | ||
| + | ===Benny=== | ||
| + | *sequenced ETFA/B col5. with Vf and Vr | ||
| + | *sequenced 21-25 col1. with Vf and Vr | ||
| + | *digested 21-25, ETFA/B with X/P | ||
| + | *run digest on a gel | ||
Latest revision as of 03:33, 29 October 2008
David
- digested:
pUC57-pTET-BisdA with SphI+PstI with CIP treatment pTET-BisdB with XbaI+PstI laqIQ-ERE-TetR with XbaI+PstI
- set up the following ligations with the gel purified products:
pUC57-pTET-BisdA+pTET-BisdB pSB1A3+laqIQ-ERE-TetR
- overnight at 16 degrees C.
TOm
- sequenced ETFA/B col5. with reverse universal
Benny
- sequenced ETFA/B col5. with Vf and Vr
- sequenced 21-25 col1. with Vf and Vr
- digested 21-25, ETFA/B with X/P
- run digest on a gel
