Team:Freiburg/Parts

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All of our parts feature our expanded Pre- and Suffix for in frame cloning of fusion proteins (more). A complete, detailed list of all parts submitted to the registry in this year´s project can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg here], as well as the respective DNA-Sequences.

basic parts

• Signal peptide
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157001 BBa_K157001]
Description:
This sequence mediates transportation of the protein to a translocational pore by an RNA–multiprotein complex, the signal recognition particle (SRP) when fused to the n-terminus of a fusion protein with a transmembrane region[1]. There, after a pause of translation, the signal sequence is released and translation and translocation of the nascent chain are restarted[2].
Source:
Human EGFR (ErbB-1) signal sequence; originally mediating membrane integration of the EGF-receptor (ErbB-1). Sequence taken from UniProtKB/Swiss-Prot entry P00533.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
References:
[1] Walter P, Johnson AE: “Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.” Annu Rev Cell Biol 1994, 10:87-119
[2] Robert M Stroud, Peter Walter: “Signal sequence recognition and protein targeting”, Current Opinion in Structural Biology 1999, 9:754–759
AA sequence:
MRPSGTAGAA LLALLAALCP ASRA
gene sequence:
ATGAGACCAT CTGGTACTGC TGGAGCCGCA TTGCTGGCAC TTTTGGCTGC GCTGTGCCCT GCAAGCAGAG CA

• Erbb1-transmembrane region
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157002 BBa_K157002]
Description:
Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
source:
Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533. Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
ATGMVGALLL LLVVALGIGL FM
gene sequence:
ATAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATG

• Sc-Fv anti NIP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157003 BBa_K157003]
Description:
Singlechain Fv.Fragment of the Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1]Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986
[2]D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988
AASequence:
QVQLQQPGAE LVKPGASVKL SCKASGYTFT SYWMHWVKQR PGRGLEWIGR IDPNSGGTKY NEKFKSKATL TVDKPSSTAY MQLSSLTSED SAVYYCARYD YYGGSYFDYW GQGTTVTVSS GGGGSGGGGS GGGGSQAVVT QESALTTSPG ETVTLTCRSS TGAVTTSNYA NWVQEKPDHL FTGLIGGTNN RAPGVPARFS GSLIGDKAAL TITGAQTEDE AIYFCALWYS NHWVFGGGTK LTVLG
gene sequence:
CAGGTGCAGC TCCAGCAGCC CGGAGCCGAA CTGGTGAAGC CAGGCGCCAG CGTGAAGCTG TCCTGCAAGG CCAGCGGCTA CACCTTCACC AGCTACTGGA TGCACTGGGT GAAACAGAGG CCCGGCAGAG GCCTGGAATG GATCGGCCGG ATCGACCCCA ACAGCGGCGG CACCAAGTAC AACGAGAAGT TCAAGAGCAA GGCCACCCTG ACCGTGGACA AGCCCAGCAG CACCGCCTAC ATGCAGCTGT CCAGCCTGAC CAGCGAGGAC AGCGCCGTGT ACTACTGCGC CAGATACGAC TACTACGGCG GCAGCTACTT CGACTACTGG GGCCAGGGCA CCACCGTGAC CGTGTCCTCT GGGGGAGGGG GCTCAGGAGG AGGAGGAAGC GGGGGAGGGG GCAGCCAGGC CGTGGTGACC CAGGAAAGCG CCCTGACCAC TCCCCTGGCG GACAGTGACC TGACCTGCCG TCCTCTACAG GCGCCGTGAC CACAAGCAAC TACGCCAACT GGGTGCAGGA AAAGCCCGAC CACCTGTTCA CCGGCCTGAT CGGCGGCACA AACAACAGAG CCCCTGGCGT GCCCGCTAGA TTCAGCGGCA GCCTGATCGG GGACAAGGCC GCCCTGACAA TCACAGGCGC CCAGACCGAG GACGAGGCCA TCTACTTTTG CGCCCTGTGG TACAGCAACC ACTGGGTGTT CGGCGGAGGG ACCAAGCTGA CCGTGCTGGG C

• LipocalinFluA
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157004 BBa_K157004]
Description:
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra[1,2].
Source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1] Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry
[2]Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)
AA sequence:
MAGDVYHDGA CPEVKPVDNF DWSQYHGKWW EVAKYPSPNG KYGKCGWAEY TPEGKSVKVS RYDVIHGKEY FMEGTAYPVG DSKIGKIYHS RTVGGYTRKT VFNVLSTDNK NYIIGYSCRY DEDKKGHWDH VWVLSRSMVL TGEAKTAVEN YLIGSPVVDS QKLVYSDFSE AACKVNNTG
gene sequence:
GACGTGTACC ACGACGGCGC CTGCCCCGAA GTGAAGCCCG TGGACAACTT CGACTGGTCC CAGTACCACG GCAAGTGGTG GGAGGTGGCC AAGTACCCCA GCCCCAACGG CAAGTATGGC AAGTGCGGCT GGGCCGAGTA CACCCCCGAG GGCAAGAGCG TGAAGGTGTC CAGATACGAC GTGATCCACG GCAAAGAATA CTTCATGGAA GGCACCGCCT ACCCCGTGGG CGACAGCAAG ATCGGCAAGA TCTACCACAG CCGGACCGTG GGCGGCTACA CCAGAAAGAC CGTGTTCAAC GTGCTGTCCA CCGACAACAA GAACTACATC ATCGGCTACA GCTGCCGCTA CGACGAGGAC AAGAAGGGCC ACTGGGACCA CGTGTGGGTG CTGTCCCGGT CCATGGTGCT GACCGGCGAG GCCAAGACCG CCGTGGAGAA CTACCTGATC GGCAGCCCCG TGGTGGACAG CCAGAAACTG GTGTACAGCG ACTTCAGCGA GGCCGCCTGC AAAGTGAACA AC

• Split-Cerulean-C-CFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157005 BBa_K157005]
Description:
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP “Cerulean”, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGSVQ LADHYQQNTP IGDGPVLLPD NHYLSTQSKL SKDPNEKRDH
gene sequence:
MVLLEFVTAA GITLGMDELY KAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAG

• Split-Cerulean-N-CFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157006 BBa_K157006]
Description:
N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP “Cerulean”, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTLGVQCFA RYPDHMKRHD FFKSAMPEGY VQERTIFFKD DGNYKTRAEV KFEGDTLVNR IELKGIDFKE DGNILGHKLE YNAISDNVYI TADKQ
gene sequence:
ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA GCTGGACGGC GACGTGAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AAGGCGACGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC TGGGCGTGCA GTGCTTCGCC AGATACCCCG ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACACT GGTGAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAATA TCCTGGGCCA CAAGCTGGAA TACAACGCCA TCAGCGACAA CGTGTACATC ACCGCCGACA GCAG

• Split-Venus-C-YFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157007 BBa_K157007]
Description:
C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP “Venus”, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGGVQ LADHYQQNTP IGDGPVLLPD NHYLSYQSKL SKDPNEKRDH MVLLEFVTAA GITHGMDELY K
gene sequence:
AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACA TCGAGGACGG CGGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GAGCAAGCTG TCCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTTGT GACAGCCGCC GGAATCACCC ACGGCATGGA CGAGCTGTAC AAG

• Split-Venus-N-YFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157008 BBa_K157008]
Description:
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKLICT TGKLPVPWPT LVTTLYLQCF ARYPDHMKQH DFFKSAMPEG YVQERTIFFK DDGNYKTRAE VKFEGDTLVN RIELKGIDFK EDGNILGHKL EYNYNSHNVY ITADKQ
gene sequence:
ATGGTGTCCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAGCTGGAC GGCGACGTGA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAAGGCGA CGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTGTACCT CCAGTGCTTC GCCAGATACC CCGACCACAT GAAGCAGCAC GATTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG AACGGACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CAGAGCCGAA GTGAAGTTCG AGGGCGACAC ACTGGTGAAC CGGATCGAGC TGAAGGGCAT CGACTTCAAA GAGGACGGCA ATATCCTGGG CCACAAGCTG GAATACAACT ACAACAGCCA CAACGTGTAC ATCACCGCCG ACAAGCAG

Source (all split-fluorophores):
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References (all split-fluorophores):
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
-[4]Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001
-[5]Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002

• Split-Fluo-Linker
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157009 BBa_K157009]
description:
Originally, this linker was used for fusion to the N-terminus of the C-terminal half of split fluorophores: Protein interactions can be examined by BiFC[1] using complementary, non-fluorescent fragments of fluorophores[2]. Therefore, it is essential that the C-terminal fragment of the fluorophore is not restricted too much in its mobility. The linker allows orientation and adaption of the C-terminal fragment to the N-terminal fragment of the split fluorophore and, thus, the reassembly of a working fluorescent protein. It has already been used in BiFC assays and is known to serve this purpose well[3]; anyway, another, more flexible linker that could be used instead is our “GGGGS-linker” (Part Bba_K157010).
References:
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA Sequence:
RPACKIPNDL KQKVMNH
gene sequence:
CGACCAGCCT GTAAGATTCC AAATGACCTG AAGCAGAAAG TTATGAATCA C

• GGGGS-LINKER_BCR-Transmem.
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157010 BBa_K157010]
Description:
Helical single-span transmembrane region of the B-Cell-Receptor with a flexible 15 aa Linker at the N-terminus. Designed for fusion to proteins or peptides that are to be presented at the cells surface; signal peptide for membrane integration (e.g. part Bba_K157001) required at the N-terminus of the whole construct!

• His-Tag (improved)
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157011 BBa_K157011]
See Part BBa_I757013 (Freiburg 2007)

• Strep-Tag II (improved)
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012 BBa_K157012]
See Part BBa_I757014 (Freiburg 2007)

• GGGS-linker (pMA)
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157013 BBa_K157013]
Description:
A flexible, 15 aa long linker designed for fusion to/of proteins or peptides via in frame cloning. Consists of Glycine and Serine only.
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
GGGSGGGSGG GSGGG
gene sequence:
GGTGGAGGAG GTTCTGGAGG CGGTGGAAGT GGTGGCGGAG GTAGC

composite parts

• Tm-Fluo-linker-cYFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157014 BBa_K157014]
Description:
C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGGVQLAD HYQQNTPIGD GPVLLPDNHY LSYQSKLSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGACG GCGGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CACGGCATGG ACGAGCTGTA CAAGACCGGT TAA

• Tm-nYFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157015 BBa_K157015]
Description:
N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGMV SKGEELFTGV VPILVELDGD VNGHKFSVSG EGEGDATYGK LTLKLICTTG KLPVPWPTLV TTLYLQCFAR YPDHMKQHDF FKSAMPEGYV QERTIFFKDD GNYKTRAEVK FEGDTLVNRI ELKGIDFKED GNILGHKLEY NYNSHNVYIT ADKQTG*
gene sequence:
ATGGCCGGCA TAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATGA CCGGCATGGT GTCCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAGC TGGACGGCGA CGTGAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAA GGCGACGCCA CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT GACCACCCTG TACCTCCAGT GCTTCGCCAG ATACCCCGAC CACATGAAGC AGCACGATTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACACTGG TGAACCGGAT CGAGCTGAAG GGCATCGACT TCAAAGAGGA CGGCAATATC CTGGGCCACA AGCTGGAATA CAACTACAAC AGCCACAACG TGTACATCAC CGCCGACAAG CAGACCGGTT AA

• Tm-Fluolinker-cCFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157016 BBa_K157016]
Description:
C-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSKLSKD PNEKRDHMVL LEFVTAAGIT LGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAGACCGGT TAA

• Tm-nCFP
  

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157017 BBa_K157017]
Description:
N-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGMS KGEELFTGVV PILVELDGDV NGHKFSVSGE GEGDATYGKL TLKFICTTGK LPVPWPTLVT TLGVQCFARY PDHMKRHDFF KSAMPEGYVQ ERTIFFKDDG NYKTRAEVKF EGDTLVNRIE LKGIDFKEDG NILGHKLEYN AISDNVYITA DKQTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAGCTGGA CGGCGACGTG AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAAGGCG ACGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTGGGCG TGCAGTGCTT CGCCAGATAC CCCGACCACA TGAAGCGGCA CGATTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CACTGGTGAA CCGGATCGAG CTGAAGGGCA TCGACTTCAA AGAGGACGGC AATATCCTGG GCCACAAGCT GGAATACAAC GCCATCAGCG ACAACGTGTA CATCACCGCC GACAAGCAGA CCGGTTAA

• Tm-betaLactamaseBla1

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157018 BBa_K157018]
Description:
First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of the parts BBa_K157002, BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGHP ETLAKVKDAE DQLGARVGYI ELDLNSGKIL ESFRPEERFP MMSTFKVLLC GAVLSRIDAG QEQLGRRIHY SQNDLVEYSP VTEKHLTDGM TVGELCSAAI TMSDNTAANL LLTTIGGPKE LTAFLRNMGD HVTRLDRWEP ELNEAIPNDE RDTTTPVAMA TTLRKLLTGE LLGTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCACCCG GAAACCCTGG CCAAAGTGAA AGATGCGGAA GATCAGCTGG GTGCGCGTGT GGGCTATATT GAACTGGATC TGAACAGCGG CAAAATTCTG GAATCTTTTC GTCCGGAAGA ACGTTTTCCG ATGATGAGCA CCTTTAAAGT GCTGCTGTGC GGTGCGGTTC TGAGCCGTAT TGATGCGGGC CAGGAACAGC TGGGCCGTCG TATTCATTAT AGCCAGAACG ATCTGGTGGA ATATAGCCCG GTGACCGAAA AACATCTGAC CGATGGCATG ACCGTGGGCG AACTGTGCAG CGCGGCGATT ACCATGAGCG ATAACACCGC GGCGAACCTG CTGCTGACCA CCATTGGCGG TCCGAAAGAA CTGACCGCGT TTCTGCGTAA CATGGGCGAT CATGTGACCC GTCTGGATCG TTGGGAACCG GAACTGAACG AAGCGATTCC GAACGATGAA CGTGATACCA CCACCCCGGT GGCCATGGCG ACCACCCTGC GTAAACTGCT GACCGGCGAA CTGCTGGGCA CCGGTTAA

• Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157019 BBa_K157019]
Description:
Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGGT PASRQQLMDW MKADKVAGPL LRSVLPAGWF IADKSGAGER GSRGIIAALG PDGKPSRIVV IYTTGSQATM DELNRQIAEI GASLIKHWTG *
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCGGTACT CCGGCTTCCC GGCAACAATT AATGGACTGG ATGAAAGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG TGCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACTGA ATCGTCAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGACCGGT TAA

• Tm-Split-Luciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157020 BBa_K157020]
Description:
Part 1 of the Split- Luciferase(BB057) fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGEA IVDIPEIPGF KDLEPMEQFI AQVDLCVDCT TGCLKGLANV QCSDLLKKWL PQRCATFASK IQGQVDKIKG AGGDTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCGAAGCG ATTGTGGATA TTCCGGAAAT TCCGGGCTTT AAAGATCTGG AACCGATGGA ACAGTTTATT GCGCAGGTGG ATCTGTGCGT GGATTGCACC ACCGGCTGCC TGAAAGGCCT GGCCAACGTG CAGTGCAGCG ATCTGCTGAA AAAATGGCTG CCGCAGCGTT GCGCGACCTT TGCGAGCAAA ATTCAGGGCC AGGTGGATAA AATTAAAGGC GCGGGTGGCG ATACCGGTTA A

• Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157021 BBa_K157021]
Description:
Second part of Split-Luciferase(BB058) fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of the parts BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGKP TENNEDFNIV AVASNFATTD LDADRGKLPG KKLPLEVLKE MEANARKAGC TRGCLICLSH IKCTPKMKKF IPGRCHTYEG DKESAQGGIG TG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCAAACCG ACCGAAAACA ACGAAGATTT TAACATTGTG GCGGTGGCGA GCAACTTTGC GACCACCGAT CTGGATGCGG ATCGTGGCAA ACTGCCGGGC AAAAAACTGC CGCTGGAAGT GCTGAAAGAA ATGGAAGCGA ACGCGCGTAA AGCCGGTTGC ACCCGTGGCT GCCTGATTTG CCTGAGCCAT ATTAAATGCA CCCCGAAAAT GAAAAAATTT ATCCCGGGTC GTTGCCATAC CTATGAAGGC GATAAAGAAA GCGCGCAGGG CGGCATTGGC ACCGGTTAA

• SP-AntiNIP-GGGSlinker

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157022 BBa_K157022]
Description:
The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGTT AA

• SP-Lipocalin-GGGSlinker

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157023 BBa_K157023]
Description:
Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGTTAA

• SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157024 BBa_K157024]
Description:
A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase. Part was derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGHP ETLAKVKDAE DQLGARVGYI ELDLNSGKIL ESFRPEERFP MMSTFKVLLC GAVLSRIDAG QEQLGRRIHY SQNDLVEYSP VTEKHLTDGM TVGELCSAAI TMSDNTAANL LLTTIGGPKE LTAFLRNMGD HVTRLDRWEP ELNEAIPNDE RDTTTPVAMA TTLRKLLTGE LLGTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCACCCG GAAACCCTGG CCAAAGTGAA AGATGCGGAA GATCAGCTGG GTGCGCGTGT GGGCTATATT GAACTGGATC TGAACAGCGG CAAAATTCTG GAATCTTTTC GTCCGGAAGA ACGTTTTCCG ATGATGAGCA CCTTTAAAGT GCTGCTGTGC GGTGCGGTTC TGAGCCGTAT TGATGCGGGC CAGGAACAGC TGGGCCGTCG TATTCATTAT AGCCAGAACG ATCTGGTGGA ATATAGCCCG GTGACCGAAA AACATCTGAC CGATGGCATG ACCGTGGGCG AACTGTGCAG CGCGGCGATT ACCATGAGCG ATAACACCGC GGCGAACCTG CTGCTGACCA CCATTGGCGG TCCGAAAGAA CTGACCGCGT TTCTGCGTAA CATGGGCGAT CATGTGACCC GTCTGGATCG TTGGGAACCG GAACTGAACG AAGCGATTCC GAACGATGAA CGTGATACCA CCACCCCGGT GGCCATGGCG ACCACCCTGC GTAAACTGCT GACCGGCGAA CTGCTGGGCA CCGGTTAA

• SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157025 BBa_K157025]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGKP TENNEDFNIV AVASNFATTD LDADRGKLPG KKLPLEVLKE MEANARKAGC TRGCLICLSH IKCTPKMKKF IPGRCHTYEG DKESAQGGIG TG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCGGTACT CCGGCTTCCC GGCAACAATT AATGGACTGG ATGAAAGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG TGCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACTGA ATCGTCAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGACCGGT TAA

• SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157026 BBa_K157026]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGGVQLAD HYQQNTPIGD GPVLLPDNHY LSYQSKLSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKTG *
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGACG GCGGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CACGGCATGG ACGAGCTGTA CAAGACCGGT TAA

• SP-AntiNIP-GGGSlinker-Tm-nYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157027 BBa_K157027]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGMV SKGEELFTGV VPILVELDGD VNGHKFSVSG EGEGDATYGK LTLKLICTTG KLPVPWPTLV TTLYLQCFAR YPDHMKQHDF FKSAMPEGYV QERTIFFKDD GNYKTRAEVK FEGDTLVNRI ELKGIDFKED GNILGHKLEY NYNSHNVYIT ADKQTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCATGGTG TCCAAGGGCG AGGAACTGTT CACCGGCGTG GTGCCCATCC TGGTGGAGCT GGACGGCGAC GTGAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAAG GCGACGCCAC CTACGGCAAG CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTGGTG ACCACCCTGT ACCTCCAGTG CTTCGCCAGA TACCCCGACC ACATGAAGCA GCACGATTTC TTCAAGAGCG CCATGCCCGA GGGCTACGTG CAGGAACGGA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCAGAGC CGAAGTGAAG TTCGAGGGCG ACACACTGGT GAACCGGATC GAGCTGAAGG GCATCGACTT CAAAGAGGAC GGCAATATCC TGGGCCACAA GCTGGAATAC AACTACAACA GCCACAACGT GTACATCACC GCCGACAAGC AGACCGGTTA A

• SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157028 BBa_K157028]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSKLSKD PNEKRDHMVL LEFVTAAGIT LGMDELYKTG *
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAGACCGGT TAA

• SP-AntiNIP-GGGSlinker-Tm-nCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157029 BBa_K157029]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and intracellular the N-terminal part of the split fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGMS KGEELFTGVV PILVELDGDV NGHKFSVSGE GEGDATYGKL TLKFICTTGK LPVPWPTLVT TLGVQCFARY PDHMKRHDFF KSAMPEGYVQ ERTIFFKDDG NYKTRAEVKF EGDTLVNRIE LKGIDFKEDG NILGHKLEYN AISDNVYITA DKQTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAGCTGGA CGGCGACGTG AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAAGGCG ACGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTGGGCG TGCAGTGCTT CGCCAGATAC CCCGACCACA TGAAGCGGCA CGATTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CACTGGTGAA CCGGATCGAG CTGAAGGGCA TCGACTTCAA AGAGGACGGC AATATCCTGG GCCACAAGCT GGAATACAAC GCCATCAGCG ACAACGTGTA CATCACCGCC GACAAGCAGA CCGGTTAA</s pan>

• SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157030 BBa_K157030]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058). Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGKP TENNEDFNIV AVASNFATTD LDADRGKLPG KKLPLEVLKE MEANARKAGC TRGCLICLSH IKCTPKMKKF IPGRCHTYEG DKESAQGGIG TG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCAAACCG ACCGAAAACA ACGAAGATTT TAACATTGTG GCGGTGGCGA GCAACTTTGC GACCACCGAT CTGGATGCGG ATCGTGGCAA ACTGCCGGGC AAAAAACTGC CGCTGGAAGT GCTGAAAGAA ATGGAAGCGA ACGCGCGTAA AGCCGGTTGC ACCCGTGGCT GCCTGATTTG CCTGAGCCAT ATTAAATGCA CCCCGAAAAT GAAAAAATTT ATCCCGGGTC GTTGCCATAC CTATGAAGGC GATAAAGAAA GCGCGCAGGG CGGCATTGGC ACCGGTTAA

• SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157031 BBa_K157031]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058). Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY YGGSYFDYWG QGTTVTVSSG GGGSGGGGSG GGGSQAVVTQ ESALTTSPGE TVTLTCRSST GAVTTSNYAN WVQEKPDHLF TGLIGGTNNR APGVPARFSG SLIGDKAALT ITGAQTEDEA IYFCALWYSN HWVFGGGTKL TVLGTGGGGG SGGGGSGGGG STGIATGMVG ALLLLLVVAL GIGLFMTGEA IVDIPEIPGF KDLEPMEQFI AQVDLCVDCT TGCLKGLANV QCSDLLKKWL PQRCATFASK IQGQVDKIKG AGGDTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCCAG GTGCAGCTCC AGCAGCCCGG AGCCGAACTG GTGAAGCCAG GCGCCAGCGT GAAGCTGTCC TGCAAGGCCA GCGGCTACAC CTTCACCAGC TACTGGATGC ACTGGGTGAA ACAGAGGCCC GGCAGAGGCC TGGAATGGAT CGGCCGGATC GACCCCAACA GCGGCGGCAC CAAGTACAAC GAGAAGTTCA AGAGCAAGGC CACCCTGACC GTGGACAAGC CCAGCAGCAC CGCCTACATG CAGCTGTCCA GCCTGACCAG CGAGGACAGC GCCGTGTACT ACTGCGCCAG ATACGACTAC TACGGCGGCA GCTACTTCGA CTACTGGGGC CAGGGCACCA CCGTGACCGT GTCCTCTGGG GGAGGGGGCT CAGGAGGAGG AGGAAGCGGG GGAGGGGGCA GCCAGGCCGT GGTGACCCAG GAAAGCGCCC TGACCACCTC CCCTGGCGAG ACAGTGACCC TGACCTGCCG GTCCTCTACA GGCGCCGTGA CCACAAGCAA CTACGCCAAC TGGGTGCAGG AAAAGCCCGA CCACCTGTTC ACCGGCCTGA TCGGCGGCAC AAACAACAGA GCCCCTGGCG TGCCCGCTAG ATTCAGCGGC AGCCTGATCG GGGACAAGGC CGCCCTGACA ATCACAGGCG CCCAGACCGA GGACGAGGCC ATCTACTTTT GCGCCCTGTG GTACAGCAAC CACTGGGTGT TCGGCGGAGG GACCAAGCTG ACCGTGCTGG GCACCGGCGG AGGAGGTGGT TCAGGTGGTG GTGGTAGTGG AGGAGGAGGA TCCACCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCGAAGCG ATTGTGGATA TTCCGGAAAT TCCGGGCTTT AAAGATCTGG AACCGATGGA ACAGTTTATT GCGCAGGTGG ATCTGTGCGT GGATTGCACC ACCGGCTGCC TGAAAGGCCT GGCCAACGTG CAGTGCAGCG ATCTGCTGAA AAAATGGCTG CCGCAGCGTT GCGCGACCTT TGCGAGCAAA ATTCAGGGCC AGGTGGATAA AATTAAAGGC GCGGGTGGCG ATACCGGTTA A

• SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157032 BBa_K157032]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker , the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGHPE TLAKVKDAED QLGARVGYIE LDLNSGKILE SFRPEERFPM MSTFKVLLCG AVLSRIDAGQ EQLGRRIHYS QNDLVEYSPV TEKHLTDGMT VGELCSAAIT MSDNTAANLL LTTIGGPKEL TAFLRNMGDH VTRLDRWEPE LNEAIPNDER DTTTPVAMAT TLRKLLTGEL LGTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CCACCCGGAA ACCCTGGCCA AAGTGAAAGA TGCGGAAGAT CAGCTGGGTG CGCGTGTGGG CTATATTGAA CTGGATCTGA ACAGCGGCAA AATTCTGGAA TCTTTTCGTC CGGAAGAACG TTTTCCGATG ATGAGCACCT TTAAAGTGCT GCTGTGCGGT GCGGTTCTGA GCCGTATTGA TGCGGGCCAG GAACAGCTGG GCCGTCGTAT TCATTATAGC CAGAACGATC TGGTGGAATA TAGCCCGGTG ACCGAAAAAC ATCTGACCGA TGGCATGACC GTGGGCGAAC TGTGCAGCGC GGCGATTACC ATGAGCGATA ACACCGCGGC GAACCTGCTG CTGACCACCA TTGGCGGTCC GAAAGAACTG ACCGCGTTTC TGCGTAACAT GGGCGATCAT GTGACCCGTC TGGATCGTTG GGAACCGGAA CTGAACGAAG CGATTCCGAA CGATGAACGT GATACCACCA CCCCGGTGGC CATGGCGACC ACCCTGCGTA AACTGCTGAC CGGCGAACTG CTGGGCACCG GTTAA

• SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157033 BBa_K157033]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the transmembrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGGTP ASRQQLMDWM KADKVAGPLL RSVLPAGWFI ADKSGAGERG SRGIIAALGP DGKPSRIVVI YTTGSQATMD ELNRQIAEIG ASLIKHWTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CGGTACTCCG GCTTCCCGGC AACAATTAAT GGACTGGATG AAAGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGTGC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACTGAATC GTCAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GACCGGTTAA

• SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157034 BBa_K157034]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGRPA CKIPNDLKQK VMNHTGKNGI KANFKIRHNI EDGGVQLADH YQQNTPIGDG PVLLPDNHYL SYQSKLSKDP NEKRDHMVLL EFVTAAGITH GMDELYKTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CCGACCAGCC TGTAAGATTC CAAATGACCT GAAGCAGAAA GTTATGAATC ACACCGGCAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACATC GAGGACGGCG GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGA GCAAGCTGTC CAAGGACCCC AACGAGAAGC GGGACCACAT GGTGCTGCTG GAATTTGTGA CAGCCGCCGG AATCACCCAC GGCATGGACG AGCTGTACAA GACCGGTTAA

• SP-Lipocalin-GGGSlinker-Tm-nYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157035 BBa_K157035]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP. Part was derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGMVS KGEELFTGVV PILVELDGDV NGHKFSVSGE GEGDATYGKL TLKLICTTGK LPVPWPTLVT TLYLQCFARY PDHMKQHDFF KSAMPEGYVQ ERTIFFKDDG NYKTRAEVKF EGDTLVNRIE LKGIDFKEDG NILGHKLEYN YNSHNVYITA DKQTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CATGGTGTCC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAGCTGGA CGGCGACGTG AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAAGGCG ACGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTGTACC TCCAGTGCTT CGCCAGATAC CCCGACCACA TGAAGCAGCA CGATTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CACTGGTGAA CCGGATCGAG CTGAAGGGCA TCGACTTCAA AGAGGACGGC AATATCCTGG GCCACAAGCT GGAATACAAC TACAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA CCGGTTAA

• SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157036 BBa_K157036]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor CFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWS QYHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSK IGKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGE AKTAVENYLIG SPVVDSQKL VYSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGRPA CKIPNDLKQK VMNHTGKNG IKANFKIRHNI EDGSVQLAD HYQQNTPIGDG PVLLPDNHYL STQSKLSKDP NEKRDHMVLL EFVTAAGITL GMDELYKTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CCGACCAGCC TGTAAGATTC CAAATGACCT GAAGCAGAAA GTTATGAATC ACACCGGCAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACATC GAGGATGGCA GCGTGCAGCT GGCCGATCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGA GCAAGCTGTC CAAGGACCCC AACGAGAAGC GGGACCACAT GGTGCTGCTG GAATTTGTGA CAGCCGCCGG AATCACCCTG GGCATGGACG AGCTGTACAA GACCGGTTAA

• SP-Lipocalin-GGGSlinker-Tm-nCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157037 BBa_K157037]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor CFP. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGMSK GEELFTGVVP ILVELDGDVN GHKFSVSGEG EGDATYGKLT LKFICTTGKL PVPWPTLVTT LGVQCFARYP DHMKRHDFFK SAMPEGYVQE RTIFFKDDGN YKTRAEVKFE GDTLVNRIEL KGIDFKEDGN ILGHKLEYNA ISDNVYITAD KQTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CATGAGCAAG GGCGAGGAAC TGTTCACCGG CGTGGTGCCC ATCCTGGTGG AGCTGGACGG CGACGTGAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAAGGCGACG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT GGTGACCACC CTGGGCGTGC AGTGCTTCGC CAGATACCCC GACCACATGA AGCGGCACGA TTTCTTCAAG AGCGCCATGC CCGAGGGCTA CGTGCAGGAA CGGACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCA GAGCCGAAGT GAAGTTCGAG GGCGACACAC TGGTGAACCG GATCGAGCTG AAGGGCATCG ACTTCAAAGA GGACGGCAAT ATCCTGGGCC ACAAGCTGGA ATACAACGCC ATCAGCGACA ACGTGTACAT CACCGCCGAC AAGCAGACCG GTTAA

• SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157038 BBa_K157038]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTA GAALLALLAA LCPASRATGD VYHDGACPE VKPVDNFDWS QYHGKWWEVAK YPSPNGKYGK CGWAEYTPEG KSVKVSRYDV IHGKEYFME GTAYPVGDSK IGKIYHSRTVG GYTRKTVFNV LSTDNKNYII GYSCRYDEDK KGHWDHVWV LSRSMVLTGE AKTAVENYLIG SPVVDSQKLV YSDFSEAACK VNNTGGGGGS GGGGSGGGG STGIATGMVG ALLLLLVVALG IGLFMTGEAI VDIPEIPGFK DLEPMEQFIA QVDLCVDCT TGCLKGLANV QCSDLLKKWLP QRCATFASKI QGQVDKIKGA GGDTG*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CGAAGCGATT GTGGATATTC CGGAAATTCC GGGCTTTAAA GATCTGGAAC CGATGGAACA GTTTATTGCG CAGGTGGATC TGTGCGTGGA TTGCACCACC GGCTGCCTGA AAGGCCTGGC CAACGTGCAG TGCAGCGATC TGCTGAAAAA ATGGCTGCCG CAGCGTTGCG CGACCTTTGC GAGCAAAATT CAGGGCCAGG TGGATAAAAT TAAAGGCGCG GGTGGCGATA CCGGTTAA

• SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157039 BBa_K157039]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of Splitluciferase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
MAGMRPSGTAG AALLALLAA LCPASRATGD VYHDGACPEV KPVDNFDWSQ YHGKWWEVAK YPSPNGKYGKC GWAEYTPEG KSVKVSRYDV IHGKEYFMEG TAYPVGDSKI GKIYHSRTVG GYTRKTVFNVL STDNKNYII GYSCRYDEDK KGHWDHVWVL SRSMVLTGEA KTAVENYLIG SPVVDSQKLVY SDFSEAACK VNNTGGGGGS GGGGSGGGGS TGIATGMVGA LLLLLVVALG IGLFMTGKPTE NNEDFNIVA VASNFATTDL DADRGKLPGK KLPLEVLKEM EANARKAGCT RGCLICLSHIK CTPKMKKFI PGRCHTYEGD KESAQGGIGT G*
gene sequence:
ATGGCCGGCA TGAGACCATC TGGTACTGCT GGAGCCGCAT TGCTGGCACT TTTGGCTGCG CTGTGCCCTG CAAGCAGAGC AACCGGCGAC GTGTACCACG ACGGCGCCTG CCCCGAAGTG AAGCCCGTGG ACAACTTCGA CTGGTCCCAG TACCACGGCA AGTGGTGGGA GGTGGCCAAG TACCCCAGCC CCAACGGCAA GTATGGCAAG TGCGGCTGGG CCGAGTACAC CCCCGAGGGC AAGAGCGTGA AGGTGTCCAG ATACGACGTG ATCCACGGCA AAGAATACTT CATGGAAGGC ACCGCCTACC CCGTGGGCGA CAGCAAGATC GGCAAGATCT ACCACAGCCG GACCGTGGGC GGCTACACCA GAAAGACCGT GTTCAACGTG CTGTCCACCG ACAACAAGAA CTACATCATC GGCTACAGCT GCCGCTACGA CGAGGACAAG AAGGGCCACT GGGACCACGT GTGGGTGCTG TCCCGGTCCA TGGTGCTGAC CGGCGAGGCC AAGACCGCCG TGGAGAACTA CCTGATCGGC AGCCCCGTGG TGGACAGCCA GAAACTGGTG TACAGCGACT TCAGCGAGGC CGCCTGCAAA GTGAACAACA CCGGCGGAGG AGGTGGTTCA GGTGGTGGTG GTAGTGGAGG AGGAGGATCC ACCGGCATAG CTACCGGAAT GGTGGGTGCA CTTTTGCTCC TTTTGGTCGT TGCCCTGGGG ATAGGACTCT TTATGACCGG CAAACCGACC GAAAACAACG AAGATTTTAA CATTGTGGCG GTGGCGAGCA ACTTTGCGAC CACCGATCTG GATGCGGATC GTGGCAAACT GCCGGGCAAA AAACTGCCGC TGGAAGTGCT GAAAGAAATG GAAGCGAACG CGCGTAAAGC CGGTTGCACC CGTGGCTGCC TGATTTGCCT GAGCCATATT AAATGCACCC CGAAAATGAA AAAATTTATC CCGGGTCGTT GCCATACCTA TGAAGGCGAT AAAGAAAGCG CGCAGGGCGG CATTGGCACC GGTTAA

• Transfection_Vector-(BBa_J52017)-CMV

Part Name:
[http://partsregistry.org/Part:BBa_K157040 BBa_K157040]
Description:
Transfection vector with CMV Promotor for expression in eukaryotic cells.

• Transfection_Vector-(BBa_J52017)-CMV-CFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157041 BBa_K157041]
Description:
Transfectionvector with CMV promoter and CFP as reporter.