Team:Rice University/Notebook/14 June 2008

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**'''Result'''-both plates showed bacterial growth at all but one spot after 24h.  The one spot appears to be a lysogen.
**'''Result'''-both plates showed bacterial growth at all but one spot after 24h.  The one spot appears to be a lysogen.
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[[image:20080614_30*C_VCS_lysogen.jpg]] [[image:20080614_42*C_VCS_lysogen.jpg]]
 
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[[image:20080614_30*C_VCS_lysogen.jpg|350px]] [[image:20080614_42*C_VCS_lysogen.jpg|350px]]
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The upper left spot on both plates is a negative control used to gauge aseptic technique.  The only colony that appears to have grown @ 30*C, but not at 42*C was the third from the right at the top.  This colony will be further screened for presence of a lysogen.
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*David Ouyang
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**PCR amplified the side tail fiber region (stf) and excision/integration segment (attp).
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**Ran gel - looks good, sent out for sequencing.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"

Latest revision as of 15:43, 27 June 2008

Saturday 14 June

  • Taylor Stevenson
    • Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
    • Result-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.


20080614 30*C VCS lysogen.jpg 20080614 42*C VCS lysogen.jpg

The upper left spot on both plates is a negative control used to gauge aseptic technique. The only colony that appears to have grown @ 30*C, but not at 42*C was the third from the right at the top. This colony will be further screened for presence of a lysogen.



  • David Ouyang
    • PCR amplified the side tail fiber region (stf) and excision/integration segment (attp).
    • Ran gel - looks good, sent out for sequencing.







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