Team:NTU-Singapore/Notebook/26 June 2008

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(New page: =Thursday 26 June= ==10:30am to 12:30pm:== *Min-Lin, Hung: miniprep (with new kit) for: **GFP self-ligated plasmid (control) **GFP producer E0480 *Choon Kit: nanodrop for digested Fe, GFP ...)
 
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=Thursday 26 June=
=Thursday 26 June=
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==10:30am to 12:30pm:==
==10:30am to 12:30pm:==
*Min-Lin, Hung: miniprep (with new kit) for:
*Min-Lin, Hung: miniprep (with new kit) for:
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**LacI-GFP: using homemade top10
**LacI-GFP: using homemade top10
*Min-Lin: inoculation (2 tubes for each plate) for pFe , pT7, Terminator, empty plasmid with AmpR, RBS32, RBS34, GFP 0240, LacI-GFP (in BL21), LacI.
*Min-Lin: inoculation (2 tubes for each plate) for pFe , pT7, Terminator, empty plasmid with AmpR, RBS32, RBS34, GFP 0240, LacI-GFP (in BL21), LacI.
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Latest revision as of 02:02, 28 October 2008

Contents

Thursday 26 June

10:30am to 12:30pm:

  • Min-Lin, Hung: miniprep (with new kit) for:
    • GFP self-ligated plasmid (control)
    • GFP producer E0480
  • Choon Kit: nanodrop for digested Fe, GFP to evaluate the amount for ligation.

2pm to 4pm:

  • Choon Kit: ligation of pFE and GFP (with some new improvements), using 2 pFe:GFP ratios (1:4 and 1:7)
  • Hung:
    • Prepare digestion mixture for GFP self-ligated and GFP E0480
    • Loading gel for GFP self-ligated, GFP E0480, Fe-GFP (1:4) and Fe-GFP (1:7)

4:30pm to 7pm:

  • Choon Kit, Hung: transformation + cloning for:
    • Fe-GFP (1:4) and Fe-GFP (1:7) : using commercial top 10
    • LacI-GFP: using homemade top10
  • Min-Lin: inoculation (2 tubes for each plate) for pFe , pT7, Terminator, empty plasmid with AmpR, RBS32, RBS34, GFP 0240, LacI-GFP (in BL21), LacI.
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/26_June_2008"