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Team:Chiba/Calendar-Home/31 August 2008
From 2008.igem.org
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:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]''' | :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]''' | ||
::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5 | ::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5 | ||
| - | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | + | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2) |
<table width="600" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="600" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample</td> | <td width="257">Sample</td> | ||
| - | <td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion | + | <td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002(1)</td><td>Single Digesiton T9002(2)</td><td>Double Digestion T9002(1)(2)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 81: | Line 81: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>XbaI(μL)</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI(μL)</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 113: | Line 113: | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | + | <td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2) |
</td><td>Single & Double Digestion</td> | </td><td>Single & Double Digestion</td> | ||
</tr> | </tr> | ||
| Line 134: | Line 134: | ||
:From Left | :From Left | ||
::*Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5 | ::*Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5 | ||
| - | ::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | + | ::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2) |
::*Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5 | ::*Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5 | ||
| - | ::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | + | ::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2) |
| - | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | + | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2) |
|} | |} | ||
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[[Team:Chiba/protocol/digestion|Digestion]] | [[Team:Chiba/protocol/digestion|Digestion]] | ||
| - | *[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | + | *[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1) |
| - | *[http://partsregistry.org/Part:BBa_J52008 BBa_J52008] | + | *[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2) |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 156: | Line 156: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI</td> |
<td>1</td><td>0</td> | <td>1</td><td>0</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>PstI</td> |
<td>1</td><td>1</td> | <td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>XbaI</td> |
<td>0</td><td>1</td> | <td>0</td><td>1</td> | ||
</tr> | </tr> | ||
| Line 191: | Line 191: | ||
-->left for 3 hours at 37 °C. | -->left for 3 hours at 37 °C. | ||
| - | --> | + | -->(1)20μl refine |
| - | --> | + | -->(2)20μl refine |
Latest revision as of 20:41, 29 October 2008
30 August 2008 <|> 1 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- Competent Cells : XL10Gold
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
- ---> We saved these plates with a refrigerator.
- Digestion
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1~3 4 Sample DNA(μL) 12 10 PstⅠ(μL) 0.2 0.2 XbaⅠ(μL) 0.2 - SpeⅠ(μL) - 0.4 Buffer 2(μL) - 1.5 Buffer 3(μL) 2 - BSA(μL) 2 1.5 dH2O(μL) 3.6 1.4 TOTAL(μL) 20 15
- --->(1/9)Gel Check
- (30/8)--->Mini prep
- --->Digestion test
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
Sample Single Digesiton 2~5 Double Digestion 2~5 Single Digestion T9002(1) Single Digesiton T9002(2) Double Digestion T9002(1)(2) Sample DNA(μL) 1 3 3 1 3 XbaI(μL) 0.1 0.1 0.1 0.1 0.1 SpeI(μL) - 0.1 - - 0.1 Buffer 2(μL) 0.9 0.8 0.9 0.9 0.8 BSA(μL) 1 1 1 1 1 dH2O(μL) 7 5 5 7 5 TOTAL(μL) 10 10 10 10 10
- --->Gel Check
Sample No. [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2) Single & Double Digestion Sample DNA(μL) 1 10 Loading Dye(μL) 1 2 dH2O(μL) 4 - TOTAL(μL) 6 12 - From Left
- Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
- Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
- Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
- Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
- From Left
Team:Output
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)
Sample No. 1 2 DNA 10 10 SpeI 1 0 PstI 1 1 XbaI 0 1 BSA(x100) 0.5 0.5 Buffer2 5 0 Buffer3 0 5 dH2O 28.5 28.5 TOTAL 50μl 50μl -->left for 3 hours at 37 °C.
-->(1)20μl refine
-->(2)20μl refine
