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Alberta NINT/24 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/23_June_2008 | < Previous entry]] | + | [[Alberta_NINT/23_June_2008 | < Previous entry]] | [[Alberta_NINT/25_June_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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Cut out desired fragment and gel purified | Cut out desired fragment and gel purified | ||
Counted and recorded colonies from yesterdays ligation: | Counted and recorded colonies from yesterdays ligation: | ||
| - | Negative | + | Negative 200 |
| - | K102007 | + | K102007 200 |
From results it seems ligation didn't work. | From results it seems ligation didn't work. | ||
Quantified K102003/B+N. | Quantified K102003/B+N. | ||
| - | + | [[Image:annotated4.jpg]] | |
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Latest revision as of 22:53, 11 October 2008
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lab work (SD):
Transformation results: 2 colonies on K102008 plate and 2 colonies on K102008 negative control plate
Redid ligation of K102002/B+N with TA0/B+N (1/100) and transformation into XL1-B cells.
Made overnights of the colonies from 23/06/08 transformation.
Made glycerol stock of K102002.
lab work (JD):
Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C
Ran gel slowly on 2% agarose
Cut out desired fragment and gel purified
Counted and recorded colonies from yesterdays ligation:
Negative 200
K102007 200
From results it seems ligation didn't work.
Quantified K102003/B+N.
