Team:Chiba/Calendar-Home/13 September 2008

From 2008.igem.org

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==Laboratory work==
==Laboratory work==
===Team:Input===
===Team:Input===
 +
Time Delay Test
Time Delay Test
-
 
+
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
#[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](BW)
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**transformants
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#Plac-RhlI -Amp(BW)
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receiver
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#Plac-LasI -Amp(BW)
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#[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)
-
#Ptet-LuxI -Amp(BW)
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<BR>sender
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#Plac-RhlI (JW1908)-Amp
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*pre-culture
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#Plac-LasI (JW1908)-Amp
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#LB-Kan 2ml培養
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#Ptet-LuxI (JW1908)-Amp
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2,3:LB-Amp -0.2%グルコース 2ml培養
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-
 
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4LB-Amp　2ml培養
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-
 
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-
-->over night
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-
 
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*本培
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-
 
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-
50ml遠心管に(1)の菌液100μlとLB-Kanを20mlを加える。
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-
 
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-
50ml遠心管に(2)、(3)それぞれの菌液10μlとLB-Amp 0.2%グルコースをそれぞれに6mlを加える。
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-
 
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-
50ml遠心管に(4)のLB-Ampを6mlを加える。
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-
 
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-
OD>2となったらWash
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-
 
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-
 
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*(1)に関して
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-
 
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(1)                                 
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-
 
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-->冷却遠心(3700rpm,3min)　　　　　　　　　　
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-
 
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上澄みを捨てる                             
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-
 
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LB-Kanを20ml加える。　　　　　　　　　　　　
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-
 
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-
 
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*(2)~(4)に関して                                 
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-
 
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(2),(3),(4)
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-
 
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-->冷却遠心(3700rpm,3min)
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-
 
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上澄みを捨てる。
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-
 
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LBを2ml加える。　　　　　　　　　　　　
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-
 
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:::-->冷却遠心(3700rpm,3min)
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-
 
+
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上澄みを捨てる。                                           
+
-
 
+
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LBを2ml加える。
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-
 
+
-
:::-->冷却遠心(3700rom,3min)
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-
 
+
-
上澄み捨てる。
+
-
 
+
-
LB-Ampを6ml加える。
+
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</tr>
</tr>
</table>
</table>
 +
 +
 +
 +
 +
 +
===Team:Communication===
===Team:Communication===
:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
-
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
 
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
 +
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
 +
{|align="justify"
{|align="justify"
|[[Image:Chiba-0913.JPG]]
|[[Image:Chiba-0913.JPG]]
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</table>
</table>
:From left;
:From left;
-
::
+
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
-
::
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
 +
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
|}
|}
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<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td><td>③</td><td>④</td>
+
<td>1</td><td>2</td><td>3</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
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</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ(&mu;L)</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.5</td><td>o.5</td><td>-</td><td>-</td>
<td>0.5</td><td>o.5</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ(&mu;L)</td>
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<td>SpeI(&mu;L)</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
</tr>
</tr>
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</tr>
</tr>
</table>
</table>
 +
 +
 +
--->(9/14)[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
===Team:Output===
===Team:Output===
no work
no work

Latest revision as of 23:52, 29 October 2008

>Home | Notebook

12 September 2008 <|> 14 September 2008

Contents

Laboratory work

Team:Input

Time Delay Test

receiver

  1. [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)


sender

  1. Plac-RhlI (JW1908)-Amp
  2. Plac-LasI (JW1908)-Amp
  3. Ptet-LuxI (JW1908)-Amp


48-well plate(Deep Well)

1:2ml 2:2ml,AHL2:2ml3:2ml4:2ml-
- -----
1:1ml,2:1ml,IPTG:2μl 1:100μl,2:1ml,IPTG:1.1μl110μl,21ml,IPTG:101μl1:1μl,21ml,IPTG:1.001μl1:1ml,2:100μl,IPTG:1.1μl1:1ml,2:10μl,IPTG:1.01μl
- -----
1:1ml,3:1ml,IPTG:2μl 1:100μl,3:1ml,IPTG:1.1μl1:10μl,3:1ml,IPTG:1.01μl1:1μl,3:1ml,IPTG1:1ml,3:100μl,IPTG:1.1μl1:1ml,3:10μl,IPTG:1.01μl
- -----
1:1ml,4:1ml,IPTG:2μl 1:100μl,4:1ml,IPTG:1.1μl1:10μl,4:1ml,IPTG:1.0μl1:1μl,4:1ml,IPTG:1.001μl1:1ml,4:100μl,IPTG:1.0μl1:1ml,4:10μl,IPTG1.01μl
- -----




Team:Communication

Gel Check
  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Chiba-0913.JPG
Sample No. 1~3
Sample DNA(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;
  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)


-->Digestion

  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)


Sample No. 1234
Sample DNA(μL) 5511
EcoRI(μL) 0.5o.5--
SpeI(μL) 0.50.5--
Buffer 1(μL) 11--
Buffer 3(μL) --1-
BSA(μL) 11--
dH2O(μL) 227.5-
TOTAL(μL) 1010101


--->(9/14)[[1]]

Team:Output

no work

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