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Team:Chiba/Calendar-Home/13 September 2008
From 2008.igem.org
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===Team:Communication=== | ===Team:Communication=== | ||
:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | :'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007) | ::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007) | ||
| - | |||
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) | ::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) | ||
| + | ::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) | ||
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{|align="justify" | {|align="justify" | ||
|[[Image:Chiba-0913.JPG]] | |[[Image:Chiba-0913.JPG]] | ||
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:From left; | :From left; | ||
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) |
| + | ::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) | ||
|} | |} | ||
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<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI(μL)</td> |
<td>0.5</td><td>o.5</td><td>-</td><td>-</td> | <td>0.5</td><td>o.5</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI(μL)</td> |
<td>0.5</td><td>0.5</td><td>-</td><td>-</td> | <td>0.5</td><td>0.5</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
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| + | --->(9/14)[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]] | ||
===Team:Output=== | ===Team:Output=== | ||
no work | no work | ||
Latest revision as of 23:52, 29 October 2008
12 September 2008 <|> 14 September 2008
Contents |
Laboratory work
Team:Input
Time Delay Test
- time-delay test
- transformants
receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)
sender
- Plac-RhlI (JW1908)-Amp
- Plac-LasI (JW1908)-Amp
- Ptet-LuxI (JW1908)-Amp
48-well plate(Deep Well)
| 1:2ml | 2:2ml,AHL | 2:2ml | 3:2ml | 4:2ml | - |
| - | - | - | - | - | - |
| 1:1ml,2:1ml,IPTG:2μl | 1:100μl,2:1ml,IPTG:1.1μl | 110μl,21ml,IPTG:101μl | 1:1μl,21ml,IPTG:1.001μl | 1:1ml,2:100μl,IPTG:1.1μl | 1:1ml,2:10μl,IPTG:1.01μl |
| - | - | - | - | - | - |
| 1:1ml,3:1ml,IPTG:2μl | 1:100μl,3:1ml,IPTG:1.1μl | 1:10μl,3:1ml,IPTG:1.01μl | 1:1μl,3:1ml,IPTG | 1:1ml,3:100μl,IPTG:1.1μl | 1:1ml,3:10μl,IPTG:1.01μl |
| - | - | - | - | - | - |
| 1:1ml,4:1ml,IPTG:2μl | 1:100μl,4:1ml,IPTG:1.1μl | 1:10μl,4:1ml,IPTG:1.0μl | 1:1μl,4:1ml,IPTG:1.001μl | 1:1ml,4:100μl,IPTG:1.0μl | 1:1ml,4:10μl,IPTG1.01μl |
| - | - | - | - | - | - |
Team:Communication
- Gel Check
- [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
|
|
-->Digestion
- [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
| Sample No. | 1 | 2 | 3 | 4 |
| Sample DNA(μL) | 5 | 5 | 1 | 1 |
| EcoRI(μL) | 0.5 | o.5 | - | - |
| SpeI(μL) | 0.5 | 0.5 | - | - |
| Buffer 1(μL) | 1 | 1 | - | - |
| Buffer 3(μL) | - | - | 1 | - |
| BSA(μL) | 1 | 1 | - | - |
| dH2O(μL) | 2 | 2 | 7.5 | - |
| TOTAL(μL) | 10 | 10 | 10 | 1 |
--->(9/14)[[1]]
Team:Output
no work
