Team:Hawaii/Notebook/2008-10-28

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:* Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
:* Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
::* Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
::* Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
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[[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125820 transformants under blue light.  Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.]]
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===Checked for Transformants===
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:<strong>Krystle</strong>
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:*BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C
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===Inoculate for Tri-Parental Conjugation===
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:<strong>Krystle</strong>
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:*Inoculated 10ml cultures of LB with
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:** BB-pRL1383a with sp<sub>100</sub>
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:** BB-pRL1383a/K125820 with sp<sub>100</sub>
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:** RP1 with kan<sub>50</sub>
== Drylab Work ==
== Drylab Work ==

Latest revision as of 03:41, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Antibiotic test

Grace
  • Ran HindIII/BamHI digested J33207 on gel
  • Extracted from gel
  • Ligated with HindIII/BamHI digested pRL1383a
  • Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
  • Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Plate of BB-pRL1383a/K125820 transformants under blue light. Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.

Checked for Transformants

Krystle
  • BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC

Inoculate for Tri-Parental Conjugation

Krystle
  • Inoculated 10ml cultures of LB with
    • BB-pRL1383a with sp100
    • BB-pRL1383a/K125820 with sp100
    • RP1 with kan50

Drylab Work

Project page

Grace
  • Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace
  • Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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