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Virginia/26 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Goals== *small broth of plated terminator *large broth of E0240(RFP) and I715039(GFP) *mini prepe PRE RFP and RET GFP for another ligation *redigest 6 parts for the three ligations *pu...) |
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*redigest 6 parts for the three ligations | *redigest 6 parts for the three ligations | ||
*purify digestions | *purify digestions | ||
| - | *run gels to verify parts | + | *run NuSieve gels to verify parts |
*three runs of 3A assembly | *three runs of 3A assembly | ||
**RFP+GFP | **RFP+GFP | ||
| Line 17: | Line 17: | ||
*previous trials of 3A assembly failed | *previous trials of 3A assembly failed | ||
**digestion should have been fine so either ligation or purification steps went wrong | **digestion should have been fine so either ligation or purification steps went wrong | ||
| - | *iGEM agreed to send us CRUCIAL non functioning part from last | + | *iGEM agreed to send us CRUCIAL non functioning part from last year's library |
**great success!! | **great success!! | ||
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| + | |||
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| + | [[Team:Virginia/Notebook|Notebook]] | ||
Latest revision as of 15:35, 27 June 2008
Goals
- small broth of plated terminator
- large broth of E0240(RFP) and I715039(GFP)
- mini prepe PRE RFP and RET GFP for another ligation
- redigest 6 parts for the three ligations
- purify digestions
- run NuSieve gels to verify parts
- three runs of 3A assembly
- RFP+GFP
- Promoter+RBS
- BP1+terminator
Notes
- previous trials of 3A assembly failed
- digestion should have been fine so either ligation or purification steps went wrong
- iGEM agreed to send us CRUCIAL non functioning part from last year's library
- great success!!
