"
Team:Paris/Modeling/Workflow Example
From 2008.igem.org
(Difference between revisions)
| (8 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Paris/Menu}} | {{Paris/Menu}} | ||
| - | {{Paris/Header|Characterization Approach : Application of our Generic Workflow | + | {{Paris/Header|Characterization Approach : Application of our Generic Workflow - an Example}} |
| + | {{Paris/Section_contents_characterization}} | ||
| - | Here, we explain the generic protocol that leads to modeling "by characterization", | + | Here, we explain the generic protocol that leads to modeling "by characterization", throught one of the parts of our system. The chosen example is '''the activity of the promoter ''pFliL'' ''' (that leads, in the [[Team:Paris/Analysis#Oscillations|core system]], to the production of the '''Fluorescent Protein FP1''') '''in function of the transcription factors ''FlhDC'' and ''FliA'' '''.<br> |
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | ||
As it is illustrated below; starting from ''molecular reactions'' '''(1)''', we develop a ''model of our system'' '''(2)''', and then we identify the ''parameters'' '''(3)''' that need to be estimated. Essentially, it amounts to estimate ''Promoters Activities as functions of their Transcription Factors''. To do so, we propose ''constructs'' '''(4)''', ''protocols'' '''(5)''' and ''programs'' '''(6)'''. | As it is illustrated below; starting from ''molecular reactions'' '''(1)''', we develop a ''model of our system'' '''(2)''', and then we identify the ''parameters'' '''(3)''' that need to be estimated. Essentially, it amounts to estimate ''Promoters Activities as functions of their Transcription Factors''. To do so, we propose ''constructs'' '''(4)''', ''protocols'' '''(5)''' and ''programs'' '''(6)'''. | ||
<br> | <br> | ||
| - | <center><span style="color: red; font-weight: bold; font-size: | + | <center><span style="color: red; font-weight: bold; font-size: 28">CLICK on the images below for further information on each step!</span></center> |
<br> | <br> | ||
<center> | <center> | ||
| Line 19: | Line 20: | ||
<html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#All_Algorithm"><img src="https://static.igem.org/mediawiki/2008/3/31/Goal.jpg" width=480></a></html> | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#All_Algorithm"><img src="https://static.igem.org/mediawiki/2008/3/31/Goal.jpg" width=480></a></html> | ||
|<html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization"><img src="https://static.igem.org/mediawiki/2008/f/fd/Principles.jpg" width=410></a></html><br> | |<html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization"><img src="https://static.igem.org/mediawiki/2008/f/fd/Principles.jpg" width=410></a></html><br> | ||
| - | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization"><img src="https://static.igem.org/mediawiki/2008/5/52/Plasmid.jpg" width=410></a></html><br> | + | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization#II-Plasmid_for_promoter_characterization"><img src="https://static.igem.org/mediawiki/2008/5/52/Plasmid.jpg" width=410></a></html><br> |
| - | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization"><img src="https://static.igem.org/mediawiki/2008/7/78/HypothesisCharact.jpg" width=410></a></html><br> | + | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Protocol_Of_Characterization#Specific_Assumptions_for_the_Experiments"><img src="https://static.igem.org/mediawiki/2008/7/78/HypothesisCharact.jpg" width=410></a></html><br> |
<html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#Parameters_Finder_Programs"><img src="https://static.igem.org/mediawiki/2008/7/78/Graph.jpg" width=410></a></html><br> | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#Parameters_Finder_Programs"><img src="https://static.igem.org/mediawiki/2008/7/78/Graph.jpg" width=410></a></html><br> | ||
<html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#All_Algorithms"><img src="https://static.igem.org/mediawiki/2008/8/83/FullSystem.jpg" width=410></a></html> | <html><a href = "https://2008.igem.org/Team:Paris/Modeling/Implementation#All_Algorithms"><img src="https://static.igem.org/mediawiki/2008/8/83/FullSystem.jpg" width=410></a></html> | ||
| Line 31: | Line 32: | ||
<div style="text-align: center"> | <div style="text-align: center"> | ||
| - | {{Paris/Toggle|Details on the Steps|Team:Paris/Modeling/Rubriques}} | + | {{Paris/Toggle|Details on the Steps|Team:Paris/Modeling/Rubriques|250px}} |
</div> | </div> | ||
| Line 39: | Line 40: | ||
[[Team:Paris/Modeling/Characterization_Approach| <Back - to the "Presentation" ]]|[[Team:Paris/Modeling/Characterization_Approach_Conclu| Next - to the "Conclusion"> ]] | [[Team:Paris/Modeling/Characterization_Approach| <Back - to the "Presentation" ]]|[[Team:Paris/Modeling/Characterization_Approach_Conclu| Next - to the "Conclusion"> ]] | ||
</center> | </center> | ||
| + | |||
| + | {{Paris/Navig|Team:Paris/Modeling/Workflow_Example}} | ||
Latest revision as of 05:16, 30 October 2008
|
Characterization Approach : Application of our Generic Workflow - an Example
Other pages:
Here, we explain the generic protocol that leads to modeling "by characterization", throught one of the parts of our system. The chosen example is the activity of the promoter pFliL (that leads, in the core system, to the production of the Fluorescent Protein FP1) in function of the transcription factors FlhDC and FliA .
By estimating all parameters of every function involved in our Full System, we achieve a "Modular Virtual Lab" of our System with potential predictive capacity.
↓ Details on the Steps ↑
|
