Team:BrownTwo/Limiter/altdesign

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Alternative construction and design possiblities
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==Examples of Additional Control Systems==
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*up-limiter
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The synthetic transcriptional control system that we have added to the registry is a powerful tool, as its elements could be put together in an enormous variety of ways.  These are some possibilities that we have drawn up:
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*dual limiter
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*limiter device as a regulatory bridge between two pathways
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*killswitch
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*Smolke’s gal2 knockout scheme for using the GAL1 promoter
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*Finding additional inducible/repressible promoters to use in the system
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*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
 
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In order to modify our limiter to regulate an endogenous gene network, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene.
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*up-limiter - upregulates a gene when the expression falls below a certain level
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A handful of these parts were available in the Registry, but we found it necessary to
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*middle-manager- a combination limiter and up-limiter, it keeps a gene's expression between two threshold levels
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*dual limiter - regulates two genes simultaneously based on the induction of one.
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Modularity –
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*limiter device as a regulatory bridge between two pathways- the induction of one gene causes threshold regulation of another
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*directed evolution for transcriptional phenotypes - express a cell-death gene if the level of induction a given gene is above or below a threshold level
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Stipulation:  If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.
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modifications to current scheme:
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Possible further modifications to these transcription factors include the use of different regulation domains,
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Involves the consideration of multiple binding sites
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Latest revision as of 06:05, 30 October 2008



Examples of Additional Control Systems

The synthetic transcriptional control system that we have added to the registry is a powerful tool, as its elements could be put together in an enormous variety of ways. These are some possibilities that we have drawn up:


  • up-limiter - upregulates a gene when the expression falls below a certain level
  • middle-manager- a combination limiter and up-limiter, it keeps a gene's expression between two threshold levels
  • dual limiter - regulates two genes simultaneously based on the induction of one.
  • limiter device as a regulatory bridge between two pathways- the induction of one gene causes threshold regulation of another
  • directed evolution for transcriptional phenotypes - express a cell-death gene if the level of induction a given gene is above or below a threshold level
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