Team:Cambridge/Signalling/Constructs

From 2008.igem.org

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==AgrA==
==AgrA==
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<pre>atgaattcgc ggccgcttct agagATGGAA ATCGCACTGG CTACGGACAA TCCATATGAG GTTCTGGAAC AGGCTAAAAA CATGAACGAC ATCGGCTGCT ATTTCCTGGA TATTCAGCTG AGCACCGACA TCAACGGTAT CAAACTGGGC TCTGAAATCC GTAAACATGA TCCGGTTGGT AACATTATTT TCGTGACTTC TCATTCTGAG CTGACCTATC TGACGTTCGT ATACAAGGTT GCAGCCATGG ATTTCATCTT TAAAGACGAT CCGGCAGAGC TGCGTACGCG CATCATCGAC TGTCTGGAAA CTGCGCACAC GCGCCTGCAA CTGCTGTCCA AAGATAACTC CGTGGAAACC ATTGAACTGA AACGTGGCAG CAACAGCGTT TACGTTCAGT ACGATGATAT CATGTTCTTC GAAAGCTCCA CTAAGTCCCA CCGTCTGATC GCGCATCTGG ATAATCGTCA GATCGAGTTC TATGGCAACC TGAAAGAGCT GAGCCAGCTG GACGATCGCT TCTTCCGTTG TCATAACTCC TTCGTTGTTA ACCGTCACAA CATCGAATCC ATCGACTCCA AAGAGCGTAT TGTATACTTC AAGAACAAAG AACACTGTTA TGCGTCTGTA CGCAATGTCA AGAAAATCTA Atactagtag cggccgctgc aggcggc</pre>
+
<code>atgaattcgc ggccgcttct agagATGGAA ATCGCACTGG CTACGGACAA TCCATATGAG GTTCTGGAAC AGGCTAAAAA CATGAACGAC ATCGGCTGCT ATTTCCTGGA TATTCAGCTG AGCACCGACA TCAACGGTAT CAAACTGGGC TCTGAAATCC GTAAACATGA TCCGGTTGGT AACATTATTT TCGTGACTTC TCATTCTGAG CTGACCTATC TGACGTTCGT ATACAAGGTT GCAGCCATGG ATTTCATCTT TAAAGACGAT CCGGCAGAGC TGCGTACGCG CATCATCGAC TGTCTGGAAA CTGCGCACAC GCGCCTGCAA CTGCTGTCCA AAGATAACTC CGTGGAAACC ATTGAACTGA AACGTGGCAG CAACAGCGTT TACGTTCAGT ACGATGATAT CATGTTCTTC GAAAGCTCCA CTAAGTCCCA CCGTCTGATC GCGCATCTGG ATAATCGTCA GATCGAGTTC TATGGCAACC TGAAAGAGCT GAGCCAGCTG GACGATCGCT TCTTCCGTTG TCATAACTCC TTCGTTGTTA ACCGTCACAA CATCGAATCC ATCGACTCCA AAGAGCGTAT TGTATACTTC AAGAACAAAG AACACTGTTA TGCGTCTGTA CGCAATGTCA AGAAAATCTA Atactagtag cggccgctgc aggcggc</code>
==AgrB==
==AgrB==
-
<pre>atgaattcgc ggccgcttct agagATGAAC TATTTTGACA ACAAGATCGA CCAATTTGCT ACTTACCTGC AAAAACGCAA CAACCTGGAC CACATCCAGT TTCTGCAAGT TCGTCTGGGC ATGCAGGTTC TGGCTAAAAA CATCGGCAAA CTGATCGTTA TGTACACCAT CGCCTATATT CTGAACATCT TCCTGTTTAC CCTGATCACC AACCTGACCT TCTATCTGAT TCGCCGTCAT GCCCACGGCG CTCATGCACC TTCTTCCTTT TGGTGTTATG TAGAGAGCAT TATTCTGTTT ATCCTGCTGC CGCTGGTAAT CGTTAACTTC CACATCAATT TCCTGATTAT GATCATCCTG ACTGTGATTT CCCTGGGTGT AATCTCTGTT TACGCTCCGG CTGCCACTAA GAAAAAGCCG ATTCCGGTGC GCCTGATCAA ACGTAAAAAG TACTACGCTA TCATCGTTTC CCTGACCCTG TTCATTATCA CCCTGATTAT CAAAGAACCG TTTGCACAGT TCATCCAGCT GGGTATTATC ATCGAAGCGA TCACTCTGCT GCCGATCTTT TTCATCAAAG AGGATCTGAA ATAAtactag tagcggccgc tgcaggcggc </pre>
+
<code>atgaattcgc ggccgcttct agagATGAAC TATTTTGACA ACAAGATCGA CCAATTTGCT ACTTACCTGC AAAAACGCAA CAACCTGGAC CACATCCAGT TTCTGCAAGT TCGTCTGGGC ATGCAGGTTC TGGCTAAAAA CATCGGCAAA CTGATCGTTA TGTACACCAT CGCCTATATT CTGAACATCT TCCTGTTTAC CCTGATCACC AACCTGACCT TCTATCTGAT TCGCCGTCAT GCCCACGGCG CTCATGCACC TTCTTCCTTT TGGTGTTATG TAGAGAGCAT TATTCTGTTT ATCCTGCTGC CGCTGGTAAT CGTTAACTTC CACATCAATT TCCTGATTAT GATCATCCTG ACTGTGATTT CCCTGGGTGT AATCTCTGTT TACGCTCCGG CTGCCACTAA GAAAAAGCCG ATTCCGGTGC GCCTGATCAA ACGTAAAAAG TACTACGCTA TCATCGTTTC CCTGACCCTG TTCATTATCA CCCTGATTAT CAAAGAACCG TTTGCACAGT TCATCCAGCT GGGTATTATC ATCGAAGCGA TCACTCTGCT GCCGATCTTT TTCATCAAAG AGGATCTGAA ATAAtactag tagcggccgc tgcaggcggc </code>
==AgrC==
==AgrC==
-
<pre>atgaattcgc ggccgcttct agagATGATT CTGATGTTCA CCATTCCAGC AATTATTAGC GGTATTAAGT ACAGCAAGCT GGATTATTTC TTCATCATCG TAATCTCTAC CCTGTCCCTG TTCCTGTTCA AAATGTTCGA TTCCGCGAGC CTGATTATCC TGACTTCCTT CATCATCATC ATGTATTTCG TAAAAATCAA ATGGTATAGC ATCCTGCTGA TCATGACCTC CCAGATTATC CTGTACTGTG CCAACTACAT GTATATCGTT ATCTACGCGT ACATCACGAA AATCTCCGAT TCTATCTTCG TTATCTTTCC GTCTTTCTTC GTGGTGTACG TTACGATCTC TATTCTGTTT TCCTATATTA TCAATCGCGT TCTGAAAAAG ATCTCTACTC CGTACCTGAT TCTGAACAAA GGTTTTCTGA TCGTCATCTC CACTATCCTG CTGCTGACCT TCTCTCTGTT CTTCTTTTAC TCTCAGATCA ACTCCGATGA GGCAAAAGTC ATCCGTCAGT ACAGCTTCAT CTTTATCGGT ATTACTATCT TCCTGAGCAT TCTGACTTTC GTTATCAGCC AGTTCCTGCT GAAAGAAATG AAGTACAAGC GCAACCAAGA AGAAATCGAG ACTTACTACG AGTACACCCT GAAGATTGAA GCGATCAACA ACGAAATGCG TAAATTTCGT CACGACTACG TTAACATTCT GACCACCCTG AGCGAATACA TCCGTGAAGA CGACATGCCT GGCCTGCGTG ATTATTTCAA CAAAAACATT GTGCCGATGA AAGATAACCT GCAAATGAAC GCTATTAAGC TGAACGGTAT CGAAAACCTG AAAGTACGTG AGATCAAGGG TCTGATTACC GCTAAAATCC TGCGTGCGCA GGAAATGAAC ATCCCAATTA GCATCGAAAT CCCAGACGAA GTTTCTAGCA TTAACCTGAA CATGATTGAC CTGTCTCGTA GCATCGGCAT CATCCTGGAC AACGCTATCG AAGCTTCCAC TGAAATCGAC GACCCGATTA TTCGCGTTGC TTTTATCGAG AGCGAAAACT CCGTGACCTT TATCGTGATG AACAAATGCG CGGACGATAT CCCGCGTATT CACGAACTGT TCCAGGAATC TTTCAGCACC AAGGGTGAAG GTCGCGGCCT GGGCCTGTCC ACCCTGAAAG AGATCGCGGA TAACGCCGAC AACGTTCTGC TGGACACTAT TATCGAAAAC GGTTTCTTCA TTCAGAAAGT CGAAATCATC AATAACTAAt actagtagcg gccgctgcag gcggc</pre>
+
<code>atgaattcgc ggccgcttct agagATGATT CTGATGTTCA CCATTCCAGC AATTATTAGC GGTATTAAGT ACAGCAAGCT GGATTATTTC TTCATCATCG TAATCTCTAC CCTGTCCCTG TTCCTGTTCA AAATGTTCGA TTCCGCGAGC CTGATTATCC TGACTTCCTT CATCATCATC ATGTATTTCG TAAAAATCAA ATGGTATAGC ATCCTGCTGA TCATGACCTC CCAGATTATC CTGTACTGTG CCAACTACAT GTATATCGTT ATCTACGCGT ACATCACGAA AATCTCCGAT TCTATCTTCG TTATCTTTCC GTCTTTCTTC GTGGTGTACG TTACGATCTC TATTCTGTTT TCCTATATTA TCAATCGCGT TCTGAAAAAG ATCTCTACTC CGTACCTGAT TCTGAACAAA GGTTTTCTGA TCGTCATCTC CACTATCCTG CTGCTGACCT TCTCTCTGTT CTTCTTTTAC TCTCAGATCA ACTCCGATGA GGCAAAAGTC ATCCGTCAGT ACAGCTTCAT CTTTATCGGT ATTACTATCT TCCTGAGCAT TCTGACTTTC GTTATCAGCC AGTTCCTGCT GAAAGAAATG AAGTACAAGC GCAACCAAGA AGAAATCGAG ACTTACTACG AGTACACCCT GAAGATTGAA GCGATCAACA ACGAAATGCG TAAATTTCGT CACGACTACG TTAACATTCT GACCACCCTG AGCGAATACA TCCGTGAAGA CGACATGCCT GGCCTGCGTG ATTATTTCAA CAAAAACATT GTGCCGATGA AAGATAACCT GCAAATGAAC GCTATTAAGC TGAACGGTAT CGAAAACCTG AAAGTACGTG AGATCAAGGG TCTGATTACC GCTAAAATCC TGCGTGCGCA GGAAATGAAC ATCCCAATTA GCATCGAAAT CCCAGACGAA GTTTCTAGCA TTAACCTGAA CATGATTGAC CTGTCTCGTA GCATCGGCAT CATCCTGGAC AACGCTATCG AAGCTTCCAC TGAAATCGAC GACCCGATTA TTCGCGTTGC TTTTATCGAG AGCGAAAACT CCGTGACCTT TATCGTGATG AACAAATGCG CGGACGATAT CCCGCGTATT CACGAACTGT TCCAGGAATC TTTCAGCACC AAGGGTGAAG GTCGCGGCCT GGGCCTGTCC ACCCTGAAAG AGATCGCGGA TAACGCCGAC AACGTTCTGC TGGACACTAT TATCGAAAAC GGTTTCTTCA TTCAGAAAGT CGAAATCATC AATAACTAAt actagtagcg gccgctgcag gcggc</code>
==AgrD==
==AgrD==
-
<pre>atgaattcgc ggccgcttct agagATGAAT ACTCTGTTCA ATCTGTTTTT CGATTTCATT ACTGGCATCC TGAAGAACAT CGGTAACATC GCAGCGTACA GCACCTGCGA CTTCATCATG GATGAAGTAG AGGTCCCGAA AGAGCTGACC CAGCTGCATG AATAAtacta gtagcggccg ctgcaggcgg c</pre>
+
<code>atgaattcgc ggccgcttct agagATGAAT ACTCTGTTCA ATCTGTTTTT CGATTTCATT ACTGGCATCC TGAAGAACAT CGGTAACATC GCAGCGTACA GCACCTGCGA CTTCATCATG GATGAAGTAG AGGTCCCGAA AGAGCTGACC CAGCTGCATG AATAAtacta gtagcggccg ctgcaggcgg c</code>
==pXyl Promoter==
==pXyl Promoter==
With BB sites: 163
With BB sites: 163
Without: 115
Without: 115
-
<pre>atgaattcgc ggccgcttct agagGAAAAA CTAAAAAAAA TATTGAAAAT ACTGATGAGG TTATTTAAGA TTAAAATAAG TTAGTTTGTT TGGGCAACAA ACTAATGTGC AACTTACTTA CAATATGACA TAAAATGCAt actagtagcg gccgctgcag atc</pre>
+
<code>atgaattcgc ggccgcttct agagGAAAAA CTAAAAAAAA TATTGAAAAT ACTGATGAGG TTATTTAAGA TTAAAATAAG TTAGTTTGTT TGGGCAACAA ACTAATGTGC AACTTACTTA CAATATGACA TAAAATGCAt actagtagcg gccgctgcag atc</code>
==pSpac Promoter==
==pSpac Promoter==
With BB sites: 155
With BB sites: 155
Without: 107
Without: 107
-
<pre>atgaattcgc ggccgcttct agagAGAACA ACCTCTGCTA AAATTCCTGA AAAATTTTGC AAAAAGTTGT TGACTTTATC TACAAGGTGT GGCATAATGT GTGGAATTGT GAGCGGATAA CAATTAAGCT Ttactagtag cggccgctgc agata</pre>
+
<code>atgaattcgc ggccgcttct agagAGAACA ACCTCTGCTA AAATTCCTGA AAAATTTTGC AAAAAGTTGT TGACTTTATC TACAAGGTGT GGCATAATGT GTGGAATTGT GAGCGGATAA CAATTAAGCT Ttactagtag cggccgctgc agata</code>
==pPac Promoter==
==pPac Promoter==
With BB sites: 125
With BB sites: 125
Without: 75
Without: 75
-
<pre>atgaattcgc ggccgcttct agagAAACGA GGTCATCATT TCCTTCCGAA AAAACGGTTG CATTTAAATC TTACATATGT AATACTTTCA AAGACTACAt actagtagcg gccgctgcag gcggc</pre>
+
<code>atgaattcgc ggccgcttct agagAAACGA GGTCATCATT TCCTTCCGAA AAAACGGTTG CATTTAAATC TTACATATGT AATACTTTCA AAGACTACAt actagtagcg gccgctgcag gcggc</code>
==pUpp Promoter==
==pUpp Promoter==
With BB sites: 285
With BB sites: 285
Without: 239
Without: 239
-
<pre>gaattcgcgg ccgcttctag agATTTTTAA AGTATGTATA CAAATGATGA ATAAATTTTG GCGATATAAT GAAGGATACA GCTCCCATAA TTGGTAAAGA TACTAGATAG ATTCATCGTA AAATCATGAT TTTGCCAAAT TTGCCCTTGA ATATTAGTAG CGTTTTCTTT ACAATCGTAA ATAGTGTAAA AAAGCGTGCA AACGCATGAA TATCATCTAA AGGAGAGATT CACATGGGAA AACTGTATGT ATTTGATCCT Ctactagtag cggccgctgc agat</pre>
+
<code>gaattcgcgg ccgcttctag agATTTTTAA AGTATGTATA CAAATGATGA ATAAATTTTG GCGATATAAT GAAGGATACA GCTCCCATAA TTGGTAAAGA TACTAGATAG ATTCATCGTA AAATCATGAT TTTGCCAAAT TTGCCCTTGA ATATTAGTAG CGTTTTCTTT ACAATCGTAA ATAGTGTAAA AAAGCGTGCA AACGCATGAA TATCATCTAA AGGAGAGATT CACATGGGAA AACTGTATGT ATTTGATCCT Ctactagtag cggccgctgc agat</code>
==consensus B. subtilis RBS==
==consensus B. subtilis RBS==
With BB sites: 54
With BB sites: 54
Without: 11
Without: 11
-
<pre>gaattcgcgg ccgcttctag agAAAGGAGG  TGTtactagt agcggccgct gcag</pre>
+
<code>gaattcgcgg ccgcttctag agAAAGGAGG  TGTtactagt agcggccgct gcag</code>
==weak B. subtilis RBS==
==weak B. subtilis RBS==
With BB sites: 54
With BB sites: 54
Without: 11
Without: 11
-
<pre>gaattcgcgg ccgcttctag agAGAGGTGG  TGTtactagt agcggccgct gcag</pre>
+
<code>gaattcgcgg ccgcttctag agAGAGGTGG  TGTtactagt agcggccgct gcag</code>
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Latest revision as of 04:05, 30 October 2008

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Lowercase letters are biobrick ends, uppercase are the actual pieces.

Contents

AgrA

atgaattcgc ggccgcttct agagATGGAA ATCGCACTGG CTACGGACAA TCCATATGAG GTTCTGGAAC AGGCTAAAAA CATGAACGAC ATCGGCTGCT ATTTCCTGGA TATTCAGCTG AGCACCGACA TCAACGGTAT CAAACTGGGC TCTGAAATCC GTAAACATGA TCCGGTTGGT AACATTATTT TCGTGACTTC TCATTCTGAG CTGACCTATC TGACGTTCGT ATACAAGGTT GCAGCCATGG ATTTCATCTT TAAAGACGAT CCGGCAGAGC TGCGTACGCG CATCATCGAC TGTCTGGAAA CTGCGCACAC GCGCCTGCAA CTGCTGTCCA AAGATAACTC CGTGGAAACC ATTGAACTGA AACGTGGCAG CAACAGCGTT TACGTTCAGT ACGATGATAT CATGTTCTTC GAAAGCTCCA CTAAGTCCCA CCGTCTGATC GCGCATCTGG ATAATCGTCA GATCGAGTTC TATGGCAACC TGAAAGAGCT GAGCCAGCTG GACGATCGCT TCTTCCGTTG TCATAACTCC TTCGTTGTTA ACCGTCACAA CATCGAATCC ATCGACTCCA AAGAGCGTAT TGTATACTTC AAGAACAAAG AACACTGTTA TGCGTCTGTA CGCAATGTCA AGAAAATCTA Atactagtag cggccgctgc aggcggc

AgrB

atgaattcgc ggccgcttct agagATGAAC TATTTTGACA ACAAGATCGA CCAATTTGCT ACTTACCTGC AAAAACGCAA CAACCTGGAC CACATCCAGT TTCTGCAAGT TCGTCTGGGC ATGCAGGTTC TGGCTAAAAA CATCGGCAAA CTGATCGTTA TGTACACCAT CGCCTATATT CTGAACATCT TCCTGTTTAC CCTGATCACC AACCTGACCT TCTATCTGAT TCGCCGTCAT GCCCACGGCG CTCATGCACC TTCTTCCTTT TGGTGTTATG TAGAGAGCAT TATTCTGTTT ATCCTGCTGC CGCTGGTAAT CGTTAACTTC CACATCAATT TCCTGATTAT GATCATCCTG ACTGTGATTT CCCTGGGTGT AATCTCTGTT TACGCTCCGG CTGCCACTAA GAAAAAGCCG ATTCCGGTGC GCCTGATCAA ACGTAAAAAG TACTACGCTA TCATCGTTTC CCTGACCCTG TTCATTATCA CCCTGATTAT CAAAGAACCG TTTGCACAGT TCATCCAGCT GGGTATTATC ATCGAAGCGA TCACTCTGCT GCCGATCTTT TTCATCAAAG AGGATCTGAA ATAAtactag tagcggccgc tgcaggcggc

AgrC

atgaattcgc ggccgcttct agagATGATT CTGATGTTCA CCATTCCAGC AATTATTAGC GGTATTAAGT ACAGCAAGCT GGATTATTTC TTCATCATCG TAATCTCTAC CCTGTCCCTG TTCCTGTTCA AAATGTTCGA TTCCGCGAGC CTGATTATCC TGACTTCCTT CATCATCATC ATGTATTTCG TAAAAATCAA ATGGTATAGC ATCCTGCTGA TCATGACCTC CCAGATTATC CTGTACTGTG CCAACTACAT GTATATCGTT ATCTACGCGT ACATCACGAA AATCTCCGAT TCTATCTTCG TTATCTTTCC GTCTTTCTTC GTGGTGTACG TTACGATCTC TATTCTGTTT TCCTATATTA TCAATCGCGT TCTGAAAAAG ATCTCTACTC CGTACCTGAT TCTGAACAAA GGTTTTCTGA TCGTCATCTC CACTATCCTG CTGCTGACCT TCTCTCTGTT CTTCTTTTAC TCTCAGATCA ACTCCGATGA GGCAAAAGTC ATCCGTCAGT ACAGCTTCAT CTTTATCGGT ATTACTATCT TCCTGAGCAT TCTGACTTTC GTTATCAGCC AGTTCCTGCT GAAAGAAATG AAGTACAAGC GCAACCAAGA AGAAATCGAG ACTTACTACG AGTACACCCT GAAGATTGAA GCGATCAACA ACGAAATGCG TAAATTTCGT CACGACTACG TTAACATTCT GACCACCCTG AGCGAATACA TCCGTGAAGA CGACATGCCT GGCCTGCGTG ATTATTTCAA CAAAAACATT GTGCCGATGA AAGATAACCT GCAAATGAAC GCTATTAAGC TGAACGGTAT CGAAAACCTG AAAGTACGTG AGATCAAGGG TCTGATTACC GCTAAAATCC TGCGTGCGCA GGAAATGAAC ATCCCAATTA GCATCGAAAT CCCAGACGAA GTTTCTAGCA TTAACCTGAA CATGATTGAC CTGTCTCGTA GCATCGGCAT CATCCTGGAC AACGCTATCG AAGCTTCCAC TGAAATCGAC GACCCGATTA TTCGCGTTGC TTTTATCGAG AGCGAAAACT CCGTGACCTT TATCGTGATG AACAAATGCG CGGACGATAT CCCGCGTATT CACGAACTGT TCCAGGAATC TTTCAGCACC AAGGGTGAAG GTCGCGGCCT GGGCCTGTCC ACCCTGAAAG AGATCGCGGA TAACGCCGAC AACGTTCTGC TGGACACTAT TATCGAAAAC GGTTTCTTCA TTCAGAAAGT CGAAATCATC AATAACTAAt actagtagcg gccgctgcag gcggc

AgrD

atgaattcgc ggccgcttct agagATGAAT ACTCTGTTCA ATCTGTTTTT CGATTTCATT ACTGGCATCC TGAAGAACAT CGGTAACATC GCAGCGTACA GCACCTGCGA CTTCATCATG GATGAAGTAG AGGTCCCGAA AGAGCTGACC CAGCTGCATG AATAAtacta gtagcggccg ctgcaggcgg c

pXyl Promoter

With BB sites: 163 Without: 115 atgaattcgc ggccgcttct agagGAAAAA CTAAAAAAAA TATTGAAAAT ACTGATGAGG TTATTTAAGA TTAAAATAAG TTAGTTTGTT TGGGCAACAA ACTAATGTGC AACTTACTTA CAATATGACA TAAAATGCAt actagtagcg gccgctgcag atc

pSpac Promoter

With BB sites: 155 Without: 107 atgaattcgc ggccgcttct agagAGAACA ACCTCTGCTA AAATTCCTGA AAAATTTTGC AAAAAGTTGT TGACTTTATC TACAAGGTGT GGCATAATGT GTGGAATTGT GAGCGGATAA CAATTAAGCT Ttactagtag cggccgctgc agata

pPac Promoter

With BB sites: 125 Without: 75 atgaattcgc ggccgcttct agagAAACGA GGTCATCATT TCCTTCCGAA AAAACGGTTG CATTTAAATC TTACATATGT AATACTTTCA AAGACTACAt actagtagcg gccgctgcag gcggc

pUpp Promoter

With BB sites: 285 Without: 239 gaattcgcgg ccgcttctag agATTTTTAA AGTATGTATA CAAATGATGA ATAAATTTTG GCGATATAAT GAAGGATACA GCTCCCATAA TTGGTAAAGA TACTAGATAG ATTCATCGTA AAATCATGAT TTTGCCAAAT TTGCCCTTGA ATATTAGTAG CGTTTTCTTT ACAATCGTAA ATAGTGTAAA AAAGCGTGCA AACGCATGAA TATCATCTAA AGGAGAGATT CACATGGGAA AACTGTATGT ATTTGATCCT Ctactagtag cggccgctgc agat

consensus B. subtilis RBS

With BB sites: 54 Without: 11 gaattcgcgg ccgcttctag agAAAGGAGG TGTtactagt agcggccgct gcag

weak B. subtilis RBS

With BB sites: 54 Without: 11 gaattcgcgg ccgcttctag agAGAGGTGG TGTtactagt agcggccgct gcag

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