Team:Cambridge/Signalling/Experiments

Experiments without links were planned but ultimately scrapped so that the team could concentrate more on building new vectors, promoters, and other standardized parts for B. subtilis. Lux was put on hold because research indicated that pLux would not work in a gram-positive chassis.

Lux system

• Testing AHL degradation in B. subtilis
• Testing AHL in E. coli
• Ligating Lux pieces
• Assembling and testing Lux receiver
• Assembling and testing Lux sender

Agr system

• Testing AGR Sender & Receiver (planned)
• PCRing and Ligating AGR pieces
• Assembling and testing AGR sender
• Assembling and testing AGR receiver