Team:University of Ottawa/27 June 2008

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(Difference between revisions)
(New page: __TOC__ '''Dan''' :'''Ligation''' ::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend: :::)
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:'''Ligation'''
:'''Ligation'''
::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
-
:::
+
:::{| class="wikitable"
 +
|-
 +
| '''Components''' || '''New Name'''
 +
|-
 +
| 0B + 0A || 1
 +
|-
 +
| 0B + 1A || 2
 +
|-
 +
| 1B + 0A || 3
 +
|-
 +
| 1B + 1A || 4
 +
|}
 +
::<li> Gel indicated that no ligation occured. There are several reasons for this:
 +
:::1. We only have a 2 bp overhang
 +
:::2.We are not using enough enzyme
 +
:::3. We did not use enough buffer
 +
::<li>Enough buffer was added and enzyme was doubled, left to react over the weekend

Revision as of 17:26, 1 July 2008

Dan

Ligation
  • Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
    • Components New Name
    0B + 0A 1
    0B + 1A 2
    1B + 0A 3
    1B + 1A 4
  • Gel indicated that no ligation occured. There are several reasons for this:
    1. We only have a 2 bp overhang
    2.We are not using enough enzyme
    3. We did not use enough buffer
  • Enough buffer was added and enzyme was doubled, left to react over the weekend
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/27_June_2008"