Revision as of 11:50, 25 October 2008

Wednesday 2 July

Arrival of E7+Imm Oligo
- PCR E7+Imm
Attempted Ligation of Fe-GFP & LacI-Rbs34
- Failed Attempt due to faulty gel
  - Loading dye was unable to keep DNA sample settled at the bottom of the well
  - Samples settled into the gel and started floating out of it.
Preliminary Speculation of problem is due to loading dye.

- miniprep for pFe,pLacI,GFP and RBS 34
- Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates).

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+<div id="header">{{User:Greenbear/sandbox/header}}</div>
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+<html>
+<div id="arrow">
+<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
+<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
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+     alt="Back to Notebook"
+     title="Back to Notebook">
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 =Wednesday 2 July=
+<div class="quote" style="margin:20px;">
+{|border="1" style="background-color:#ffffcc;" cellpadding="20"
+|
 *Arrival of E7+Imm Oligo
 **PCR E7+Imm
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 ***Samples settled into the gel and started floating out of it.
 *Preliminary Speculation of problem is due to loading dye.
-*Luchao, Hung:
+===Luchao, Hung===
 **miniprep for pFe,pLacI,GFP and RBS 34
 **Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates).
+</div>