Team:KULeuven/Tools

From 2008.igem.org

(Difference between revisions)
(→Lab Templates)
Line 14: Line 14:
  Usage: <nowiki>{{:Team:KULeuven/Tools/Navigation_Bar}}</nowiki>
  Usage: <nowiki>{{:Team:KULeuven/Tools/Navigation_Bar}}</nowiki>
===Lab Templates===
===Lab Templates===
-
[[Team:KULeuven/Tools/PCR | PCR sheet]]
+
==== [[Team:KULeuven/Tools/PCR | PCR sheet]] ====
-
Usage: ... for Jonas
+
 
 +
'''Usage'''
 +
<nowiki>
 +
{{:Team:KULeuven/Tools/PCR
 +
|tanneal=55
 +
|telong=2'30"
 +
|ncycles=30
 +
|buffer=5x 10μL
 +
|mgcl=10μM 0μL
 +
|dntp=10μM 1μL
 +
|fwprimername=6 LacZ-Fw
 +
|fwprimerconc=10μM 2.5μL
 +
|rvprimername=7 LacZ-Rv
 +
|rvprimerconc=10μM 2.5μL
 +
|water=34μL
 +
|polymerase=Taq 0.5μL
 +
|dna=0.5μL of E. coli DNA extract
 +
|size=952bp
 +
|success=YES/NO
 +
|image=pcrproducts_GE.jpg
 +
|lane=3+4
 +
}}
 +
</nowiki>
 +
 
 +
'''Result'''
 +
 
 +
{{:Team:KULeuven/Tools/PCR
 +
|tanneal=55
 +
|telong=2'30"
 +
|ncycles=30
 +
|buffer=5x 10μL
 +
|mgcl=10μM 0μL
 +
|dntp=10μM 1μL
 +
|fwprimername=6 LacZ-Fw
 +
|fwprimerconc=10μM 2.5μL
 +
|rvprimername=7 LacZ-Rv
 +
|rvprimerconc=10μM 2.5μL
 +
|water=34μL
 +
|polymerase=Taq 0.5μL
 +
|dna=0.5μL of E. coli DNA extract
 +
|size=952bp
 +
|success=YES/NO
 +
|image=pcrproducts_GE.jpg
 +
|lane=3+4
 +
}}
 +
 
[[Team:KULeuven/Tools/Digestion | Digestion sheet]]
[[Team:KULeuven/Tools/Digestion | Digestion sheet]]
  Usage: ...
  Usage: ...

Revision as of 12:41, 3 July 2008

Under Construction... 

Current issues:

Templates can't be stored within the Team namespace!
They must either be stored under the Template namespace or the User namespace.

A mail has been sent to Meagan of the IGEM team to see whether it is allowed to have a team folder under the 
Template namespace.

Contents

Templates

General

Navigation Bar 

Usage: {{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage 

 {{:Team:KULeuven/Tools/PCR
 |tanneal=55
 |telong=2'30"
 |ncycles=30
 |buffer=5x 10μL
 |mgcl=10μM 0μL
 |dntp=10μM 1μL
 |fwprimername=6 LacZ-Fw
 |fwprimerconc=10μM 2.5μL
 |rvprimername=7 LacZ-Rv
 |rvprimerconc=10μM 2.5μL
 |water=34μL
 |polymerase=Taq 0.5μL
 |dna=0.5μL of E. coli DNA extract
 |size=952bp
 |success=YES/NO
 |image=pcrproducts_GE.jpg
 |lane=3+4
 }}

Result

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet 

Usage: ...

Ligation sheet 

Usage: ...

CSS

Calendar CSS file required for notebook.

Retrieved from "http://2008.igem.org/Team:KULeuven/Tools"